EFNB1 Human

Ephrin-B1 is a single-pass type I membrane protein that contains one ephrin receptor-binding (RBD) domain . The protein is involved in bidirectional signaling, meaning it can send signals both to the cell expressing the ephrin and to the cell expressing the Eph receptor . This signaling is crucial for various biological processes, including neural crest migration, growth cone development, and cancer progression .

Ephrin-B1 plays a significant role in the developing nervous system by modulating presynaptic development, axon guidance, and cell migration . In the adult nervous system, it restricts new synapse formation in the hippocampus, influencing long-term contextual memory . Additionally, Ephrin-B1 is overexpressed in atherosclerotic tissue and is involved in the regulation of cytoskeletal dynamics through intramembrane proteolysis .

Abnormal expression of Ephrin-B1 has been linked to various cancers, including prostate cancer, where it regulates the activation of metalloproteinase-8 via increased GTP-bound ARF1 GTPase . It also mediates cell-cell adhesion in both epithelial and cancer cells .

Recombinant human Ephrin-B1 is typically produced in HEK293 cells and is available in various forms, including carrier-free and Fc chimera proteins . The recombinant protein is used in research to study its interactions with Eph receptors and its role in various biological processes .

Lyophilized recombinant Ephrin-B1 is stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solutions can be stored at 4-8°C for 2-7 days, and aliquots can be stored at < -20°C for up to 3 months .

Ephrin-B1 is a fascinating protein with diverse roles in both development and disease. Its recombinant form is a valuable tool for researchers studying cell signaling and related biological processes.