EFNB2 Mouse

Ephrin-B2 is structurally related to GPI-anchored Ephrin-A ligands. It is a transmembrane protein that interacts with EphB receptors to mediate bidirectional signaling . This interaction is essential for establishing arterial versus venous identity and for the formation of angiogenic capillary plexi . Ephrin-B2 is involved in axon guidance, neural crest cell migration, and the establishment of segmental boundaries .

Recombinant Mouse Ephrin-B2 is typically produced using mammalian cell lines such as HEK293 or mouse myeloma cell lines . The protein is expressed with a fused Fc region of human IgG1 at the C-terminus to facilitate purification and detection . The recombinant protein is then purified to a high degree of purity, often exceeding 90% as determined by SDS-PAGE .

Recombinant Ephrin-B2 is used in various research applications, including:

• Functional ELISA assays: To measure its binding ability with EphB receptors .
• Cell culture studies: To investigate its role in cell adhesion, migration, and angiogenesis .
• In vivo studies: To explore its function in neural crest cell migration and axon guidance .

Recombinant Ephrin-B2 is typically lyophilized from a sterile PBS solution and can be stored at -20°C to -80°C for up to twelve months . It is recommended to avoid repeated freeze-thaw cycles to maintain protein stability .