EFNB2 Human

Ephrin- B2 Human Recombinant
Cat. No.
BT3138
Source
E.coli.
Synonyms
Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L, EFNB2, EPLG5, HTKL, LERK5, MGC126226, MGC126227, MGC126228.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EFNB2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (28-229 a.a.) and having a molecular mass of 24.9kDa.
EFNB2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ephrin-B2 is a member of the ephrin (EPH) family, which, along with EPH-related receptors, constitute the largest subfamily of receptor protein-tyrosine kinases. These molecules play a crucial role in developmental processes, particularly in the nervous system and erythropoiesis. Ephrins are categorized into two classes based on their structure and sequence: ephrin-A (EFNA) and ephrin-B (EFNB). EFNA proteins are anchored to the membrane through a glycosylphosphatidylinositol linkage, while EFNB proteins, including Ephrin-B2, are transmembrane proteins. Ephrin-B2 specifically binds to the EPHB4 and EPHA3 receptors.
Description
Recombinant human EFNB2, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 227 amino acids (residues 28-229). It has a molecular weight of 24.9 kDa. The protein features a 25 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The EFNB2 protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 20% glycerol, and 200 mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the EFNB2 protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L, EFNB2, EPLG5, HTKL, LERK5, MGC126226, MGC126227, MGC126228.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMIVLEP IYWNSSNSKF LPGQGLVLYP QIGDKLDIIC PKVDSKTVGQ YEYYKVYMVD KDQADRCTIK KENTPLLNCA KPDQDIKFTI KFQEFSPNLW GLEFQKNKDY YIISTSNGSL EGLDNQEGGV CQTRAMKILM KVGQDASSAG STRNKDPTRR PELEAGTNGR SSTTSPFVKP NPGSSTDGNS AGHSGNNILG SEVALFA.

Product Science Overview

Structure and Function

Ephrin-B2 is a transmembrane protein that binds to Eph receptors on adjacent cells, leading to bidirectional signaling. This signaling can be divided into two types:

  • Forward signaling: Initiated by the Eph receptor.
  • Reverse signaling: Initiated by the ephrin ligand.

Ephrin-B2 binds promiscuously to several Eph receptors, including EPHA4, EPHA3, and EPHB4. This interaction is essential for cell migration, repulsion, and adhesion, which are critical for the proper formation of tissues and organs .

Role in Development

Ephrin-B2 plays a pivotal role in the development of the cardiovascular system. It is involved in heart morphogenesis and the regulation of cell adhesion and migration. Ephrin-B2, along with its receptor EPHB4, is crucial for angiogenesis, the process by which new blood vessels form from pre-existing vessels. This process is vital for supplying oxygen and nutrients to tissues during development and in response to injury .

Recombinant Human Ephrin-B2

Recombinant human Ephrin-B2 is a laboratory-produced version of the protein, often used in research to study its functions and interactions. It is typically expressed in HEK 293 cells and purified to high levels of purity, making it suitable for various applications such as SDS-PAGE, ELISA, and Western blotting .

Applications in Research

Recombinant human Ephrin-B2 is used in various research applications to understand its role in development and disease. It is particularly valuable in studying cardiovascular diseases, cancer progression, and neurodegenerative diseases. By understanding how Ephrin-B2 interacts with its receptors and other cellular components, researchers can develop potential therapeutic strategies for these conditions .

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