EFNB1 Human, Sf9
Sf9, Baculovirus cells.
Ephrin B1, ELK Ligand, Ephrin-B1, Elk-L, EPLG2, LERK2, EFL3, Craniofrontonasal Syndrome (Craniofrontonasal Dysplasia), Eph-Related Receptor Tyrosine Kinase Ligand 2, EPH-Related Receptor Tyrosine Kinase Ligand 2, LERK-2, EFL-3, CFND, EFB1, CFNS, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2.
Greater than 90% as determined by SDS-PAGE.
Description
EFNB1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 452 amino acids (28-237a.a.) and having a molecular mass of 50.3kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
EFNB1 is expressed with a 242 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Ephrin B1, ELK Ligand, Ephrin-B1, Elk-L, EPLG2, LERK2, EFL3, Craniofrontonasal Syndrome (Craniofrontonasal Dysplasia), Eph-Related Receptor Tyrosine Kinase Ligand 2, EPH-Related Receptor Tyrosine Kinase Ligand 2, LERK-2, EFL-3, CFND, EFB1, CFNS, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2.
Sf9, Baculovirus cells.
ADPLAKNLEP VSWSSLNPKF LSGKGLVIYP KIGDKLDIIC PRAEAGRPYE YYKLYLVRPE QAAACSTVLD PNVLVTCNRP EQEIRFTIKF QEFSPNYMGL EFKKHHDYYI TSTSNGSLEG LENREGGVCR TRTMKIIMKV GQDPNAVTPE QLTTSRPSKE ADNTVKMATQ APGSRGSLGD
SDGKHETVNQ EEKSGPGASG GSSGDPDGFF NSKLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN
QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Product Science Overview
Ephrin-B1 is a transmembrane protein consisting of three main domains:
- Extracellular Domain (ECD): Comprising 210 amino acids, this domain is responsible for binding to Eph receptors.
- Transmembrane Segment: A 21 amino acid segment that anchors the protein to the cell membrane.
- Cytoplasmic Domain: An 88 amino acid segment involved in intracellular signaling .
The interaction between Ephrin ligands and their Eph receptors generates bidirectional signaling, affecting both receptor and ligand-expressing cells. This signaling plays a crucial role in the regulation of neural crest migration, growth cone development, and cancer progression .
Ephrin-B1 is involved in several key biological processes:
- Nervous System Development: It modulates presynaptic development, axon guidance, and cell migration. In the adult nervous system, Ephrin-B1 restricts new synapse formation in the hippocampus, influencing long-term contextual memory .
- Cardiac Tissue Cohesion: Ephrin-B1 interacts with the claudin-5/ZO-1 complex on the lateral membrane of cardiomyocytes, ensuring proper cardiac tissue cohesion .
- Cell-Cell Adhesion: It mediates cell-cell adhesion through an Eph receptor mechanism in both epithelial and cancer cells .
Abnormal expression of Ephrin-B1 has been linked to various pathological conditions:
- Atherosclerosis: Overexpressed in atherosclerotic tissue, T lymphocytes, and macrophages in monocytes and plaques .
- Cancer: Ephrin-B1 is involved in the secretion and invasion of cancer cells, particularly in prostate cancer, by regulating the activation of metalloproteinase-8 via increased GTP-bound ARF1 GTPase .
