DKK3 Human, Sf9

Dickkopf-Related Protein 3 Human Recombinant, Sf9
Cat. No.
BT30526
Source

Sf9, Baculovirus cells.

Synonyms
Dickkopf 3 homolog (Xenopus laevis), dickkopf-related protein 3, regulated in glioma, RIG, RIG-like 7-1, RIG-like 5-6, Dkk-3, REIC.
Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DKK3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 338 amino acids (22-350a.a.) and having a molecular mass of 37.3kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
DKK3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Dickkopf-related protein 3 (DKK3), a member of the DKK protein family that also includes Dkk-1, 2, and 4, is a secreted glycoprotein. This protein, consisting of 350 amino acids, plays a role in embryonic development by inhibiting the WNT signaling pathway. Structurally, DKK3 features an N-terminal signal peptide and two cysteine-rich domains separated by a 12-amino acid linker region. Notably, DKK3 gene expression is often reduced in various cancer cell lines, suggesting its potential role as a tumor suppressor gene.
Description
Recombinant Human DKK3, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. This protein comprises 338 amino acids (22-350a.a.), resulting in a molecular mass of 37.3 kDa. However, on SDS-PAGE, the molecular size appears between 40-57 kDa. DKK3 is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The DKK3 protein solution is provided at a concentration of 0.5 mg/ml and is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of DKK3 is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Dickkopf 3 homolog (Xenopus laevis), dickkopf-related protein 3, regulated in glioma, RIG, RIG-like 7-1, RIG-like 5-6, Dkk-3, REIC.
Source

Sf9, Baculovirus cells.

Amino Acid Sequence

ADPAPAPTAT SAPVKPGPAL SYPQEEATLN EMFREVEELM EDTQHKLRSA VEEMEAEEAA AKASSEVNLA NLPPSYHNET NTDTKVGNNT IHVHREIHKI TNNQTGQMVF SETVITSVGD EEGRRSHECI IDEDCGPSMY CQFASFQYTC QPCRGQRMLC TRDSECCGDQ LCVWGHCTKM ATRGSNGTIC DNQRDCQPGL CCAFQRGLLF PVCTPLPVEG ELCHDPASRL LDLITWELEP DGALDRCPCA SGLLCQPHSH SLVYVCKPTF VGSRDQDGEI LLPREVPDEY EVGSFMEEVR QELEDLERSL TEEMALREPA AAAAALLGGE EIHHHHHH.

Product Science Overview

Gene and Protein Structure

The DKK3 gene is located on chromosome 11 in humans and is expressed in various tissues, including the endothelial cells, neural tissues, and the adrenal gland . The human recombinant DKK3 protein produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain consisting of 338 amino acids (22-350a.a.) and has a molecular mass of approximately 37.3 kDa . On SDS-PAGE, the molecular size appears to be around 40-57 kDa due to glycosylation .

Biological Functions

DKK3 is involved in the negative regulation of the Wnt signaling pathway, which is essential for various developmental processes . It acts as a tumor suppressor gene, with its expression being decreased in several cancer cell lines . The protein’s role in inhibiting the Wnt signaling pathway makes it a critical player in maintaining cellular homeostasis and preventing uncontrolled cell proliferation.

Applications and Research

Recombinant DKK3 protein is widely used in research to study its role in embryonic development, cancer biology, and other cellular processes. The human recombinant DKK3 produced in Sf9 cells is particularly valuable for in vitro studies due to its high purity and biological activity .

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