DKK3 Antibody

Mouse Anti Human Dickkopf-Related Protein 3
Cat. No.
BT16252
Source
Synonyms

Dickkopf 3 homolog (Xenopus laevis), dickkopf-related protein 3, regulated in glioma, RIG, RIG-like 7-1, RIG-like 5-6, Dkk-3, REIC.

Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Dickkopf-related protein 3 (DKK3), a member of the DKK protein family alongside Dkk-1, 2, and 4, is a secreted glycoprotein containing 350 amino acids. Its structure consists of an N-terminal signal peptide and two cysteine-rich domains, separated by a 12 amino acid linker region. DKK3 plays a role in embryonic development by inhibiting the WNT signaling pathway. Studies have shown decreased DKK3 gene expression in various cancer cell lines, suggesting its potential role as a tumor suppressor gene.
Physical Appearance
Clear and colorless solution that has been sterilized through filtration.
Formulation
The antibody is provided at a concentration of 1mg/ml in a buffer solution containing PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This DKK3 antibody has undergone testing via ELISA and Western blot analysis to confirm its specificity and reactivity. However, optimal working concentrations may vary depending on the specific application and should be determined through titration.
Synonyms

Dickkopf 3 homolog (Xenopus laevis), dickkopf-related protein 3, regulated in glioma, RIG, RIG-like 7-1, RIG-like 5-6, Dkk-3, REIC.

Purification Method

DKK3 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Type
Mouse Anti Human Monoclonal.
Clone

PAT103B5AT.

Immunogen

Anti-human DKK3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DKK3 protein (22-350 amino acids purified from E. coli.

Ig Subclass

Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Introduction

Mouse Anti Human Dickkopf-Related Protein 3 is an antibody specifically designed to target the human Dickkopf-Related Protein 3 (DKK3). This protein is a member of the Dickkopf family, which plays a crucial role in various biological processes, particularly in the Wnt signaling pathway.

Dickkopf-Related Protein 3 (DKK3)

Dickkopf-Related Protein 3 is encoded by the DKK3 gene in humans. This gene is located on chromosome 11 in humans and chromosome 7 in mice . The protein contains two cysteine-rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway .

Function and Significance

DKK3 is known to antagonize the canonical Wnt signaling pathway by inhibiting the interaction between LRP5/6 and Wnt proteins . This inhibition is crucial for various developmental processes, including antero-posterior axial patterning, limb development, somitogenesis, and eye formation . In adults, DKK3 is implicated in bone formation, bone diseases, cancer, and Alzheimer’s disease .

Role in Cancer

The expression of DKK3 is decreased in various cancer cell lines, suggesting that it may function as a tumor suppressor gene . This makes DKK3 a significant target for cancer research and therapy.

Mouse Anti Human DKK3 Antibody

The Mouse Anti Human DKK3 antibody is derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DKK3 protein . This antibody is used in various research applications to study the function and regulation of DKK3 in different biological processes and diseases.

Applications in Research

The Mouse Anti Human DKK3 antibody is widely used in research to:

  • Investigate the role of DKK3 in embryonic development and its interactions with the Wnt signaling pathway.
  • Study the involvement of DKK3 in various cancers and its potential as a tumor suppressor.
  • Explore the implications of DKK3 in bone formation, bone diseases, and neurodegenerative diseases like Alzheimer’s .

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