IP10 Guinea Pig

Chemokine (C-X-C motif) ligand 10 (CXCL10), also known as IP-10, is a small cytokine within the CXC chemokine family. Produced by various cells like monocytes, endothelial cells, and fibroblasts, CXCL10 plays diverse roles. These include attracting monocytes and T cells, facilitating T cell adhesion to endothelial cells, exhibiting antitumor activity, and inhibiting both bone marrow colony formation and angiogenesis. The gene responsible for CXCL10 resides on human chromosome 4, clustered among other CXC chemokines. CXCL10 exerts its effects by binding to the CXCR3 chemokine receptor on cell surfaces. Notably, its three-dimensional crystal structure has been thoroughly analyzed under three distinct conditions, achieving a resolution of up to 1.92A.

Recombinant Guinea Pig IP10, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 76 amino acids, resulting in a molecular weight of 8.7kDa. The purification of IP10 is achieved through proprietary chromatographic techniques.

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM Sodium Phosphate, pH 7.5.

For reconstitution, it is advised to dissolve the lyophilized IP10 in sterile water to a concentration of 0.1 mg/ml. This solution can be further diluted into other aqueous solutions as needed.

Lyophilized IP10, while stable at room temperature for up to 3 weeks, should ideally be stored in a dry environment below -18°C. Upon reconstitution, Human IP10 can be kept at 4°C for 2-7 days. For extended storage, it is recommended to freeze it below -18°C. To ensure optimal long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. It's crucial to avoid repeated freeze-thaw cycles.

CXCL10, CRG-2.

Escherichia Coli.

IPHSRTIRCT CIETSTQPVN PKSFKKLEII PASQSCPRVE IIATMKMNGE KRCLDPESKV IKNLLKAVRK ERSKRS