CHMP4A Human
Description
CHMP4A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-265) and having a molecular mass of 32.0kDa.
CHMP4A is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CHMP4A is a single, non-glycosylated polypeptide chain containing 285 amino acids and has a molecular mass of approximately 32.0 kDa . It is fused to a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques . The protein is expressed in higher quantities in skeletal muscle, kidney, liver, and heart .
CHMP4A is involved in several critical cellular processes:
- Multivesicular Body Formation: MVBs contain intraluminal vesicles (ILVs) generated by invagination and scission from the limiting membrane of the endosome. These MVBs are mostly delivered to lysosomes, enabling the degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes, and lipids .
- Endosomal Sorting: CHMP4A is essential for the sorting of endosomal cargo proteins into MVBs, which is a vital step in the degradation pathway of surface receptor proteins .
- HIV-1 Infection: During HIV-1 infection, the virus utilizes the ESCRT-III complex to facilitate the budding and exocytosis of viral proteins. CHMP4A interacts with a protein engaged by HIV-1 p6, which is present in viral Gag assembly and budding .
The recombinant form of CHMP4A is produced in Escherichia coli (E. coli) and is available as a sterile, filtered colorless solution. The formulation typically contains 20 mM Tris-HCl buffer (pH 8.0), 200 mM NaCl, 0.1 mM PMSF, 1 mM EDTA, 2 mM DTT, and 50% glycerol . The protein is stable when stored at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage. It is recommended to add a carrier protein (0.1% HSA or BSA) for extended storage periods .
CHMP4A has several applications in research and biotechnology:
- Study of Endosomal Sorting and MVB Formation: Researchers use CHMP4A to study the mechanisms of endosomal sorting and MVB formation, which are essential for understanding cellular trafficking and degradation pathways.
- HIV Research: The role of CHMP4A in HIV-1 budding and exocytosis makes it a valuable tool for studying viral replication and developing potential therapeutic interventions.
- Protein-Protein Interaction Studies: The His-tagged recombinant CHMP4A can be used in protein-protein interaction studies to identify and characterize its binding partners.
