CHMP1A Human
Chromatin Modifying Protein 1A Human Recombinant
Cat. No.
BT7908
Source
Escherichia Coli.
Synonyms
Charged multivesicular body protein 1a, Chromatin-modifying protein 1a, Vacuolar protein sorting-associated protein 46-1, Vps46-1, hVps46-1, CHMP1A, CHMP1, KIAA0047, PCOLN3, PRSM1, PCH8, VPS46A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CHMP1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (1-196 a.a.) and having a molecular mass of 24.1kDa (Molecular size on SDS-PAGE will appear higher).
CHMP1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CHMP1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Charged multivesicular body protein 1a (CHMP1A), a member of the vacuolar sorting protein family, plays a crucial role as a chromatin-modifying protein. This protein is integral to complexes responsible for the sorting of endosomal components into multivesicular bodies (MVBs) and is vital for MVB formation. The MVB pathway facilitates the distribution of transmembrane proteins into lysosomes for degradation. CHMP1A interacts with VPS4B and is localized to early endosomes.
Description
Recombinant human CHMP1A, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 219 amino acids (1-196 a.a.) with a molecular mass of 24.1 kDa. Note: The molecular size on SDS-PAGE might appear higher. This CHMP1A protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, filtered solution that is colorless.
Formulation
The CHMP1A protein solution (0.5 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 85.0% as determined by SDS-PAGE analysis.
Synonyms
Charged multivesicular body protein 1a, Chromatin-modifying protein 1a, Vacuolar protein sorting-associated protein 46-1, Vps46-1, hVps46-1, CHMP1A, CHMP1, KIAA0047, PCOLN3, PRSM1, PCH8, VPS46A.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDDTLFQ LKFTAKQLEK LAKKAEKDSK AEQAKVKKAL LQKNVECARV YAENAIRKKN EGVNWLRMAS RVDAVASKVQ TAVTMKGVTK NMAQVTKALD KALSTMDLQK VSSVMDRFEQ QVQNLDVHTS VMEDSMSSAT TLTTPQEQVD SLIMQIAEEN GLEVLDQLSQ LPEGASAVGE SSVRSQEDQL SRRLAALRN.
Product Science Overview
Structure and Localization
Functions
- Formation of Multivesicular Bodies (MVBs): CHMP1A is required for the formation of MVBs, which are involved in the sorting and degradation of membrane proteins .
- Gene Silencing: Within the nucleus, CHMP1A plays a role in stable gene silencing, contributing to the regulation of gene expression .
- Tumor Suppression: CHMP1A has been reported to function as a tumor suppressor, particularly in human pancreatic cancer cells . It mediates the anti-proliferative effects of All-trans Retinoic Acid (ATRA) by regulating Cellular Retinol-Binding Protein 1 (CRBP-1) and influencing the expression of P53 and phospho-P53 .
Clinical Significance
The tumor suppressor role of CHMP1A is particularly noteworthy in the context of pancreatic cancer, which has one of the worst prognoses among all cancers . Research has shown that CHMP1A mediates the growth inhibitory activity of ATRA in pancreatic cancer cells, suggesting its potential as a therapeutic target .
Research and Applications
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