CHMP2B Human
Chromatin Modifying Protein 2B Human Recombinant
Cat. No.
BT8052
Source
Escherichia Coli.
Synonyms
Chromatin modifying protein 2B, CHMP2.5, VPS2B, Vacuolar protein sorting-associated protein 2-2, hVps2-2, DMT1, DKFZp564O123, VPS2 homolog B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CHMP2B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-213) and having a molecular mass of 26.1 kDa.
The CHMP2B is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
The CHMP2B is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
CHMP2B, a member of the vacuolar sorting protein family, plays a crucial role in the ESCRT-III complex. This complex is vital for sorting endosomal articles into multivesicular bodies (MVBs) and is essential for their formation. CHMP2B is commonly found in various tissues including the brain, heart, skeletal muscle, small intestine, pancreas, lung, placenta, and leukocytes.
Description
Recombinant human CHMP2B, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 233 amino acids (1-213) with a molecular weight of 26.1 kDa. This protein is fused to a 20 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
CHMP2B protein is supplied at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCL (pH 8), 0.1M NaCl, 2mM DTT, and 20% Glycerol.
Stability
For short-term storage (2-4 weeks), the protein should be kept at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. It is crucial to avoid repeated freezing and thawing of the protein.
Purity
Analysis by SDS-PAGE indicates a purity greater than 90%.
Synonyms
Chromatin modifying protein 2B, CHMP2.5, VPS2B, Vacuolar protein sorting-associated protein 2-2, hVps2-2, DMT1, DKFZp564O123, VPS2 homolog B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA KIGNKEACKV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD EEIERQLKAL GVD
Product Science Overview
Structure and Expression
Function and Mechanism
The ESCRT-III complex, which includes CHMP2B, is involved in the concentration and invagination of ubiquitinated endosomal cargos into intraluminal vesicles (ILVs) . These ILVs are then delivered to lysosomes for degradation . The ESCRT machinery also plays a role in other membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses like HIV-1 .
Clinical Significance
Recombinant CHMP2B
Recombinant CHMP2B proteins are typically expressed in systems like Escherichia coli or HEK293T cells . These recombinant proteins are used in various research applications, including SDS-PAGE, mass spectrometry, and cell culture studies . They are often tagged with His or DDK tags to facilitate purification and detection .
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