CD58 Human, Sf9
Sf9, Baculovirus cells.
Lymphocyte function-associated antigen 3 isoform 1, CD58, ag3, LFA-3, LFA3, Surface glycoprotein LFA-3.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
CD58 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 196 amino acids (29-215a.a.) and having a molecular mass of 22.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CD58 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD33, a putative adhesion molecule found on myelomonocytic cells, facilitates sialic-acid-dependent cell binding, showing a preference for alpha-2,6-linked sialic acid. Interestingly, its sialic acid recognition site can be obscured by interactions with sialic acids present on the same cell surface (cis interactions). In the context of immune response, CD33 acts as an inhibitory receptor upon ligand-induced tyrosine phosphorylation. This inhibition is achieved by recruiting cytoplasmic phosphatases through its SH2 domains, leading to the dephosphorylation of signaling molecules and subsequent blockage of signal transduction. Moreover, CD33 has been shown to induce apoptosis in acute myeloid leukemia cells.
Produced in Sf9 insect cells using baculovirus expression system, our recombinant human CD58 is a single, glycosylated polypeptide chain. This protein spans 196 amino acids (29-215a.a.), resulting in a molecular mass of 22.5 kDa. Note: On SDS-PAGE, the apparent molecular size might appear between 28-40 kDa due to glycosylation. A 9 amino acid His tag is present at the C-terminus to facilitate purification, achieved through proprietary chromatographic methods.
The product is a clear, colorless solution that has been sterilized by filtration.
The CD58 protein solution is provided at a concentration of 1 mg/ml and is formulated in a phosphate buffered saline (pH 7.4) solution containing 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To further enhance long-term stability during frozen storage, the addition of a carrier protein (either 0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles to maintain product integrity.
The purity of our CD58 protein is greater than 95%, as determined by SDS-PAGE analysis.
Lymphocyte function-associated antigen 3 isoform 1, CD58, ag3, LFA-3, LFA3, Surface glycoprotein LFA-3.
Sf9, Baculovirus cells.
ADPFSQQIYG VVYGNVTFHV PSNVPLKEVL WKKQKDKVAE LENSEFRAFS SFKNRVYLDT VSGSLTIYNL TSSDEDEYEM ESPNITDTMK FFLYVLESLP SPTLTCALTN GSIEVQCMIP EHYNSHRGLI MYSWDCPMEQ CKRNSTSIYF KMENDLPQKI QCTLSNPLFN TTSSIILTTC IPSSGHSRHR HHHHHH
Product Science Overview
CD58, also known as Lymphocyte Function-associated Antigen 3 (LFA-3), is a cell adhesion molecule that plays a crucial role in the immune response. It is a member of the immunoglobulin superfamily and is primarily expressed on the surface of antigen-presenting cells, such as dendritic cells, macrophages, and B cells. CD58 interacts with CD2, a receptor found on T cells and natural killer (NK) cells, facilitating cell-cell adhesion and enhancing the immune response.
The recombinant form of CD58, specifically CD58 (Human Recombinant, Sf9), is produced using the baculovirus expression system in Sf9 insect cells. This method involves several key steps:
- Gene Cloning: The gene encoding human CD58 is cloned into a baculovirus transfer vector.
- Transfection: The recombinant baculovirus DNA is transfected into Sf9 insect cells.
- Virus Amplification: The transfected cells produce recombinant baculovirus, which is then amplified through several rounds of infection.
- Protein Expression: Sf9 cells are infected with the amplified recombinant baculovirus, leading to the expression of CD58 protein.
- Protein Purification: The expressed CD58 protein is purified using affinity chromatography techniques to obtain a high-purity product.
CD58 is involved in various biochemical interactions and signaling pathways. Its primary function is to mediate cell adhesion through its interaction with CD2. This interaction is critical for the formation of the immunological synapse, a specialized junction between T cells and antigen-presenting cells. The binding of CD58 to CD2 enhances T cell activation, proliferation, and cytokine production.
In addition to its role in immune cell adhesion, CD58 has been implicated in several pathological conditions. For example, CD58 expression is upregulated in certain cancers, such as hepatocellular carcinoma, where it promotes tumor progression and metastasis by activating the AKT/GSK-3β/β-catenin signaling pathway . Furthermore, CD58 is involved in the regulation of antiviral responses, inflammatory responses in autoimmune diseases, and immune rejection of transplantation .
