CD58 Antibody, Biotin
Description
Product Specs
Product Science Overview
CD58, also known as Lymphocyte Function-associated Antigen 3 (LFA-3), is a cell adhesion molecule that plays a crucial role in the immune response. It is primarily expressed on the surface of antigen-presenting cells and interacts with CD2 on T cells to facilitate cell adhesion and signaling.
CD58 is a glycoprotein with a molecular weight of approximately 60-70 kDa. It exists in two forms: a transmembrane form and a glycosylphosphatidylinositol (GPI)-anchored form. Both forms are involved in mediating cell-cell interactions, particularly between T cells and antigen-presenting cells. The interaction between CD58 and CD2 is essential for the formation of the immunological synapse, which is critical for effective immune responses.
The Mouse Anti-Human CD58 antibody is a monoclonal antibody that specifically targets the CD58 molecule on human cells. This antibody is conjugated with biotin, a vitamin that allows for easy detection and purification of the antibody-antigen complex. The biotinylated form of the antibody is particularly useful in various laboratory applications, including flow cytometry, immunohistochemistry, and immunoprecipitation.
- Flow Cytometry: The biotin-conjugated Mouse Anti-Human CD58 antibody is widely used in flow cytometry to analyze the expression of CD58 on the surface of cells. This application is crucial for studying immune cell interactions and identifying specific cell populations.
- Immunohistochemistry (IHC): In IHC, this antibody is used to detect CD58 expression in tissue samples. It helps in understanding the distribution and localization of CD58 in different tissues, which is important for studying various diseases and immune responses.
- Immunoprecipitation: The antibody can be used to isolate CD58 from cell lysates, allowing researchers to study its interactions with other proteins and its role in cellular signaling pathways.
The study of CD58 and its interactions with CD2 is vital for understanding the mechanisms of immune cell activation and adhesion. Research involving the Mouse Anti-Human CD58 antibody has contributed to our knowledge of immune responses, autoimmune diseases, and potential therapeutic targets for immunotherapy.
