CD58 Human
Description
CD58 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques
Product Specs
Product Science Overview
CD58 is a cell surface protein that interacts with its natural ligand, CD2, which is primarily expressed on the surface of T cells and natural killer (NK) cells . This interaction is essential for the adhesion and activation of T cells, facilitating the immune response. The binding of CD58 to CD2 enhances the formation of the immunological synapse, which is critical for effective T cell activation and subsequent immune functions .
Recombinant human CD58 is produced using advanced biotechnological methods. It is typically expressed in mammalian cell lines, such as NS0 or HEK293 cells, to ensure proper folding and post-translational modifications . The recombinant protein is often tagged with a histidine (His) tag or fused with the Fc region of human immunoglobulin G (IgG) to facilitate purification and detection .
Recombinant human CD58 has several applications in research and clinical settings:
- Immunological Studies: It is used to study T cell activation, adhesion, and immune synapse formation.
- Cell Proliferation Assays: CD58 can be used in cell proliferation assays to measure the activation and proliferation of T cells in the presence of anti-CD3 antibodies .
- Therapeutic Research: CD58 is being investigated for its potential therapeutic applications in immune-related diseases and cancer immunotherapy.
Recombinant human CD58 is typically lyophilized from a filtered solution in phosphate-buffered saline (PBS) with or without carrier proteins such as bovine serum albumin (BSA) . The lyophilized protein should be reconstituted in sterile PBS and stored under specific conditions to maintain its stability and activity .
