CD58 Antibody

CD58 exists in two isoforms:

1. Glycosylphosphatidylinositol (GPI)-linked form: This isoform is anchored to the cell membrane via a GPI linkage.
2. Transmembrane form: This isoform spans the cell membrane.

Both isoforms can be expressed on the same cell type, except for erythrocytes, which only express the GPI-linked isoform .

CD58 is widely distributed across various cell types, including both hematopoietic (blood-forming) and non-hematopoietic cells. It is expressed on endothelial cells, fibroblasts, and other cell types .

CD58 interacts with CD2, a receptor found on T cells and natural killer (NK) cells. This interaction is vital for:

• Cell adhesion: Facilitates the binding of T cells to antigen-presenting cells (APCs) and target cells.
• T-cell activation: Enhances the activation of T cells in response to antigens.
• Cell-mediated cytotoxicity: Plays a role in the destruction of target cells by cytotoxic T cells and NK cells .

The Mouse Anti-Human CD58 antibody is a monoclonal antibody that specifically binds to the CD58 antigen. It is commonly used in research and clinical applications, such as:

• Flow cytometry: For analyzing the expression of CD58 on various cell types.
• Immunohistochemistry (IHC): For detecting CD58 in tissue samples.
• Western blotting: For identifying CD58 protein in cell lysates .

The antibody is typically conjugated with fluorescent dyes like FITC (Fluorescein isothiocyanate) to facilitate detection and analysis .

The study of CD58 and its interactions with CD2 is essential for understanding immune responses, particularly in the context of T-cell activation and cell-mediated cytotoxicity. Research involving CD58 can provide insights into immune system disorders, cancer immunotherapy, and transplant rejection .