CD44 Human
CD44 Molecule (Indian Blood Group), Hematopoietic Cell E- And L-Selectin Ligand, GP90 Lymphocyte Homing/Adhesion Receptor, Chondroitin Sulfate Proteoglycan 8, Extracellular Matrix Receptor III, Heparan Sulfate Proteoglycan, Phagocytic Glycoprotein 1, Hyaluronate Receptor, Hermes Antigen, ECMR-III, HUTCH-I, Epican, CDW44, MDU2, MDU3, MIC4, LHR, CD44 Antigen (Homing Function And Indian Blood Group System),Homing Function And Indian Blood Group System, Cell Surface Glycoprotein CD44, Phagocytic Glycoprotein I, Soluble CD44, CSPG8, HCELL, PGP-1, PGP-I, MC56, Pgp1, IN, CD44 .
Greater than 95.0% as determined by SDS-PAGE.
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Description
CD44 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (21-220 a.a.) and fused to a 239 aa hIgG-His-tag at C-terminus containing a total of 439 amino acids and having a molecular mass of 49kDa.
CD44 shows multiple bands between 57-70kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Cluster of Differentiation 44 (CD44) is a cell surface glycoprotein involved in various cellular processes, including cell-cell interactions, adhesion, and migration. As a receptor for hyaluronic acid and other ligands like osteopontin, collagens, and matrix metalloproteinases, CD44 plays a crucial role in lymphocyte activation, recirculation, homing, hematopoiesis, and potentially tumor metastasis.
Recombinant CD44, expressed in Sf9 insect cells using a baculovirus system, consists of a single glycosylated polypeptide chain (amino acids 21-220) fused to a 239 amino acid hIgG-His tag at the C-terminus. The resulting protein has 439 amino acids and a molecular weight of 49 kDa. Analysis by SDS-PAGE under reducing conditions reveals multiple bands between 57-70 kDa, which is characteristic of glycosylated proteins. The protein is purified using proprietary chromatographic techniques.
The CD44 protein solution is provided at a concentration of 0.25 mg/ml in a buffer containing 10% glycerol and Phosphate Buffered Saline (PBS) at pH 7.4.
The purity of the CD44 protein is determined to be greater than 95% based on SDS-PAGE analysis.
CD44 Molecule (Indian Blood Group), Hematopoietic Cell E- And L-Selectin Ligand, GP90 Lymphocyte Homing/Adhesion Receptor, Chondroitin Sulfate Proteoglycan 8, Extracellular Matrix Receptor III, Heparan Sulfate Proteoglycan, Phagocytic Glycoprotein 1, Hyaluronate Receptor, Hermes Antigen, ECMR-III, HUTCH-I, Epican, CDW44, MDU2, MDU3, MIC4, LHR, CD44 Antigen (Homing Function And Indian Blood Group System),Homing Function And Indian Blood Group System, Cell Surface Glycoprotein CD44, Phagocytic Glycoprotein I, Soluble CD44, CSPG8, HCELL, PGP-1, PGP-I, MC56, Pgp1, IN, CD44 .
QIDLNITCRF AGVFHVEKNG RYSISRTEAA DLCKAFNSTL PTMAQMEKAL SIGFETCRYG FIEGHVVIPR IHPNSICAAN NTGVYILTSN TSQYDTYCFN ASAPPEEDCT SVTDLPNAFD GPITITIVNR DGTRYVQKGE YRTNPEDIYP SNPTDDDVSS GSSSERSSTS GGYIFYTFST VHPIPDEDSP WITDSTDRIP LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH
Product Science Overview
CD44 is encoded by the CD44 gene located on chromosome 11 in humans. The protein consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for binding to its primary ligand, hyaluronan (HA), a major component of the extracellular matrix .
CD44 exists in multiple isoforms due to alternative splicing of its mRNA. These isoforms can vary significantly in their structure and function, contributing to the protein’s versatility in various physiological and pathological processes .
CD44 is involved in a wide range of biological functions, including:
- Cell Adhesion: CD44 mediates cell-cell and cell-matrix adhesion by binding to hyaluronan and other ligands such as osteopontin, collagens, and matrix metalloproteinases .
- Lymphocyte Activation and Homing: CD44 plays a critical role in the activation and homing of lymphocytes to sites of inflammation or injury .
- Tumor Progression and Metastasis: CD44 is implicated in cancer cell proliferation, invasion, and metastasis. It is considered a marker for cancer stem cells and is involved in the epithelial-mesenchymal transition (EMT), a process crucial for cancer metastasis .
Recombinant human CD44 is produced using various expression systems, including HEK293 cells and Chinese Hamster Ovary (CHO) cells. The recombinant protein is typically purified and characterized to ensure high purity and functionality .
- Purity: Recombinant CD44 proteins are usually purified to a high degree, often exceeding 95% purity as determined by SDS-PAGE and other analytical methods .
- Activity: The biological activity of recombinant CD44 is assessed through various assays, including binding assays to its ligands such as hyaluronan .
- Applications: Recombinant CD44 is used in research to study its role in cell adhesion, migration, and signaling. It is also utilized in cancer research to investigate its function in tumor progression and metastasis .
CD44 has significant clinical implications, particularly in cancer diagnosis and therapy. It serves as a biomarker for cancer stem cells and is a potential therapeutic target for inhibiting tumor growth and metastasis . Additionally, CD44 is involved in the regulation of immune responses, making it a target for immunotherapy in various diseases .
