CD44 Anti-Human, FITC

CD44 is synthesized as a molecule with a molecular weight of 37 kilodaltons (kDa). Upon glycosylation, it is converted to an 80- to 95-kDa form. Alternatively, a 180- to 200-kDa form results from the addition of chondroitin sulfate . This glycoprotein plays a crucial role in various physiological processes, including:

• Cell Migration: CD44 is involved in the migration of cells, particularly during immune responses and wound healing.
• Lymphocyte Homing: It aids in the homing of lymphocytes to peripheral lymphoid organs and sites of inflammation.
• Cell Adhesion: CD44 facilitates the adhesion of cells to the extracellular matrix and other cells, which is essential during hematopoiesis and lymphocyte activation .

CD44 expression varies depending on the activation state of cells. For instance, as B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. High CD44 expression on regulatory T cells (Treg cells) has been associated with potent suppressive function via high production of interleukin-10 (IL-10) .

The Mouse Anti-Human CD44 Antibody is a monoclonal antibody that specifically binds to the CD44 antigen. This antibody is often conjugated with Fluorescein Isothiocyanate (FITC), a fluorescent dye that allows for the visualization of CD44 expression using techniques such as flow cytometry and immunofluorescence .

• Host Species: Mouse
• Immunogen: Normal human peripheral blood lymphocytes or dexamethasone-induced myeloid leukemia M1 cells .
• Formulation: The antibody is typically formulated in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and bovine serum albumin (BSA) .

The Mouse Anti-Human CD44 FITC-conjugated antibody is widely used in various research applications, including:

• Flow Cytometry: For the analysis of CD44 expression on different cell types.
• Immunocytochemistry: For the detection of CD44 in cultured cells.
• Immunohistochemistry: For the staining of formalin-fixed, paraffin-embedded tissue sections .

The CD44 antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light. It should not be frozen to maintain its stability and effectiveness .