CD44 Mouse Antibody

The Rat Anti-Mouse CD44 antibody is typically produced by immunizing rats with mouse CD44 antigen. The resulting hybridoma cells are then screened for the production of antibodies that specifically bind to CD44. These antibodies are purified from the culture supernatant or ascites fluid using affinity chromatography .

Some key characteristics of Rat Anti-Mouse CD44 antibodies include:

• Isotype: Rat IgG2b, κ or Rat IgG2a, κ
• Immunogen: Dexamethasone-induced, SJL mouse spontaneous myeloid leukemia M1 cells
• Applications: Flow cytometry, immunohistochemistry (formalin-fixed paraffin and acetone-fixed frozen sections), and other immunoassays

Rat Anti-Mouse CD44 antibodies are valuable tools in various research applications, including:

1. Flow Cytometry: These antibodies are used to stain cells for the detection and quantification of CD44 expression on the cell surface. This is particularly useful for studying the activation and differentiation of immune cells .

2. Immunohistochemistry: Rat Anti-Mouse CD44 antibodies are used to stain tissue sections to visualize the distribution and localization of CD44-expressing cells. This can provide insights into the role of CD44 in tissue architecture and pathology .

3. Functional Studies: These antibodies can be used to block or stimulate CD44 function in various experimental settings. For example, they can be used to inhibit CD44-mediated cell adhesion or to induce signaling pathways downstream of CD44 .