CD44 Anti-Human, Biotin

CD44 is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. It is also known by several other names, including Hermes, Pgp-1, H-CAM, and HUTCH . CD44 is expressed on a wide variety of cell types, including leukocytes, endothelial cells, hepatocytes, and mesenchymal cells . The expression of CD44 increases significantly as B and T cells become activated or progress to the memory stage, making it a valuable marker for memory cell subsets .

The preparation of CD44, Mouse Anti-Human Biotin involves several steps:

1. Immunogen Preparation: The immunogen used is typically dexamethasone-induced myeloid leukemia M1 cells .
2. Antibody Production: The antibody is produced in rats and is of the IgG2b, κ isotype .
3. Purification: The antibody is purified by affinity chromatography to ensure high purity and specificity .
4. Biotin Conjugation: The purified antibody is then conjugated with biotin under optimal conditions to facilitate its use in various applications .

CD44 functions as an adhesion molecule through its binding to hyaluronic acid, an extracellular matrix component . This interaction plays a crucial role in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs, and sites of inflammation . High CD44 expression on regulatory T cells (Treg cells) has been associated with potent suppressive function via high production of IL-10 .

The biotinylated form of the antibody allows for its use in various applications, including:

• Flow Cytometry: For immunofluorescent staining and flow cytometric analysis .
• Immunocytochemistry: For staining cells in culture .
• Immunohistochemistry: For staining tissue sections .