CD44 Antibody
CD44, Mouse Anti-Human
Cat. No.
BT574
Source
Synonyms
MDU2, MDU3, MIC4, CDW44, CSPG8, HCELL, HUTCH-I, Phagocytic glycoprotein I, PGP-1, Extracellular matrix receptor-III, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican) CDw44.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
CD44 is a cell surface glycoprotein involved in cell-cell interactions, adhesion, and migration. It acts as a receptor for hyaluronic acid and interacts with other ligands like osteopontin, collagens, and matrix metalloproteinases. CD44 participates in various cellular functions, including lymphocyte activation, recirculation, homing, hematopoiesis, and tumor metastasis. Research suggests CD44 and CD49d might be activity markers and CD44 a potential therapeutic target in multiple sclerosis. Elevated CD44 antigen levels are linked to relapses in non-small cell lung cancers.
Formulation
The antibody is provided at a concentration of 1mg/ml in PBS after reconstitution.
Storage Procedures
The lyophilized antibody should be stored at 4°C. Once reconstituted, it's recommended to use it within a month. For long-term storage, aliquot the antibody and store it at -20°C.
Solubility
To reconstitute, add the appropriate amount of H2O to the vial. Mix gently, ensuring the solution washes the sides of the vial. Allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for staining applications. Use 10µl of antibody per 1,000,000 cells.
Available Conjugates
This antibody is also available in biotin and FITC conjugates. For staining with biotin or FITC-conjugated antibody, use 5-10µl per 1 million cells.
Synonyms
MDU2, MDU3, MIC4, CDW44, CSPG8, HCELL, HUTCH-I, Phagocytic glycoprotein I, PGP-1, Extracellular matrix receptor-III, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican) CDw44.
Purification Method
Protein-A.
Type
Mouse Anti Human Monoclonal.
Clone
hCD44.
Immunogen
Purified human T-Cells.
Ig Subclass
Mouse IgG1.
Product Science Overview
Biological Properties
CD44 is involved in various biological processes, including:
- Lymphocyte Activation: CD44 is upregulated during T cell activation and plays a role in the inflammatory response .
- Cell Adhesion: It mediates the binding of lymphocytes to high endothelial venules, facilitating their migration back into lymphoid tissues .
- Tumor Progression: Interactions between CD44 and HER2 have been linked to increased ovarian carcinoma cell growth .
Expression Patterns and Tissue Distribution
CD44 is expressed in a wide range of tissues, including:
- Immune Cells: High levels are found on the surface of T cells, B cells, and macrophages .
- Epithelial Cells: It is present in various epithelial tissues, contributing to cell adhesion and migration .
- Cancer Cells: Elevated levels of CD44 are often observed in cancerous tissues, where it is associated with tumor progression and metastasis .
Biological Functions
The primary functions of CD44 include:
- Cell Migration: CD44 interacts with the actin cytoskeleton through its association with ezrin, radixin, and moesin (ERM) proteins, facilitating cell movement .
- Signal Transduction: It participates in signaling pathways that regulate cell proliferation, survival, and differentiation .
- Matrix Degradation: CD44 contributes to the degradation of the ECM, promoting tissue remodeling and repair .
Modes of Action
CD44 mediates its effects through several mechanisms:
- Ligand Binding: It binds to HA and other ECM components, initiating downstream signaling pathways .
- Protein Interactions: CD44 interacts with various intracellular proteins, linking the ECM to the cytoskeleton and influencing cell behavior .
- Phosphorylation: The phosphorylation state of CD44 regulates its interactions with other proteins and its role in cell migration .
Regulatory Mechanisms
The expression and activity of CD44 are regulated by:
- Alternative Splicing: Different splice variants of CD44 are produced in response to various physiological and pathological conditions .
- Post-Translational Modifications: Glycosylation, phosphorylation, and other modifications influence the function and localization of CD44 .
- Transcriptional Regulation: The expression of CD44 is controlled by various transcription factors and signaling pathways .
Mouse Anti-Human CD44 Antibodies
Mouse anti-human CD44 antibodies are widely used in research to study the expression and function of CD44 in human tissues. These antibodies are developed by immunizing mice with human CD44 protein, and they are used in various applications, including:
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