CD44 Antibody, Biotin

Rat Anti-Mouse CD44, Biotinylated is a monoclonal antibody used extensively in immunological research. This antibody targets the CD44 antigen, a cell-surface glycoprotein involved in various cellular functions such as cell-cell interactions, cell adhesion, and migration. The biotinylation of this antibody enhances its utility in various detection and purification assays.

CD44 is an 80-95 kD glycoprotein also known by several other names, including Hermes, Pgp-1, H-CAM, and HUTCH-1 . It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. CD44 plays a crucial role in lymphocyte activation, recirculation, and homing, as well as in hematopoiesis and tumor metastasis .

CD44 serves as a receptor for hyaluronic acid and interacts with other ligands such as osteopontin, collagens, and matrix metalloproteinases . It is involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs, and sites of inflammation, and leukocyte aggregation . High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10 .

The Rat Anti-Mouse CD44 antibody is produced by immunizing rats with CD44 enriched mouse lymph node T cells . The antibody is then purified using affinity chromatography and conjugated with biotin under optimal conditions . Biotinylation allows for easy detection and purification using streptavidin-based systems.

This biotinylated antibody is used in various applications, including:

• Flow Cytometry (FC): For immunofluorescent staining and flow cytometric analysis .
• Immunocytochemistry (ICC): For staining cells to visualize the presence and distribution of CD44 .
• Immunohistochemistry (IHC): For staining tissue sections to study CD44 expression in different tissues .

The antibody solution should be stored undiluted between 2°C and 8°C and should not be frozen . For long-term storage, aliquoting and storing at -20°C is recommended .