CD14 Human

CD14 Human Recombinant
Cat. No.
BT25442
Source
E.coli.
Synonyms
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 353 amino acids (20-349 a.a) and having a molecular mass of 37.9kDa.
CD14is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD14, also known as lipopolysaccharide (LPS) receptor, is primarily found on monocytes and macrophages, with weaker expression on neutrophils. It is a glycosylphosphatidylinositol (GPI)-anchored protein that serves as a high-affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14 can also bind LPS, acting as an agonist at physiological concentrations and an antagonist at higher concentrations in cell activation. Additionally, CD14 has been observed to bind apoptotic cells.
Description
Recombinant human CD14, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 353 amino acids (residues 20-349). It has a molecular weight of 37.9 kDa. The protein includes a 23-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that is sterile-filtered.
Formulation
The CD14 protein solution has a concentration of 0.5 mg/ml and is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 30% glycerol, 0.15 M NaCl, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSTTPEPCE LDDEDFRCVC NFSEPQPDWS EAFQCVSAVE VEIHAGGLNL EPFLKRVDAD ADPRQYADTV KALRVRRLTV GAAQVPAQLL VGALRVLAYS RLKELTLEDL KITGTMPPLP LEATGLALSS LRLRNVSWAT GRSWLAELQQ WLKPGLKVLS IAQAHSPAFS CEQVRAFPAL TSLDLSDNPG LGERGLMAAL CPHKFPAIQN LALRNTGMET PTGVCAALAA AGVQPHSLDL SHNSLRATVN PSAPRCMWSS ALNSLNLSFA GLEQVPKGLP AKLRVLDLSC NRLNRAPQPD ELPEVDNLTL DGNPFLVPGT ALPHEGSMNS GVV.

Product Science Overview

Structure and Function

The human CD14 protein is encoded by the CD14 gene and consists of 375 amino acids . It includes a 19 amino acid signal peptide and a C-terminal hydrophobic region characteristic of glycosylphosphatidylinositol (GPI)-anchored proteins . The GPI anchor allows CD14 to be attached to the cell membrane, although a soluble form of CD14 (sCD14) also exists in the serum .

CD14 enhances the immune response by binding to LPS and facilitating its recognition by the Toll-like receptor 4 (TLR4)-MD-2 complex . This interaction triggers a cascade of signaling events leading to the production of pro-inflammatory cytokines and the activation of the immune system .

Recombinant CD14

Recombinant human CD14 is produced using various expression systems, including Chinese Hamster Ovary (CHO) cells and HEK293 cells . The recombinant protein is often tagged with a His-tag to facilitate purification and detection . It is used in research to study the immune response, particularly the mechanisms of LPS recognition and signaling.

The recombinant CD14 protein is typically purified to a high degree of purity (>95%) and is tested for endotoxin levels to ensure its suitability for experimental use . It is available in both carrier-free and carrier-containing formulations, depending on the specific application requirements .

Applications

Recombinant CD14 is widely used in immunological research to:

  • Study the interaction between LPS and the TLR4-MD-2 complex.
  • Investigate the signaling pathways activated by LPS.
  • Explore the role of CD14 in various immune responses and diseases.

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