CD14 Antibody, Biotin
Description
Product Specs
Product Science Overview
Cluster of Differentiation 14 (CD14) is a protein primarily expressed on the surface of monocytes and macrophages, which are key players in the innate immune system . CD14 acts as a co-receptor along with Toll-like receptor 4 (TLR4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria . This protein exists in two forms: a membrane-bound form (mCD14) and a soluble form (sCD14) .
- Membrane-bound CD14 (mCD14): Anchored to the cell membrane by a glycosylphosphatidylinositol (GPI) tail.
- Soluble CD14 (sCD14): Found in the serum and other body fluids, either shed from the cell surface or directly secreted.
CD14 plays a crucial role in the immune response by recognizing and binding to LPS, which triggers a cascade of signaling events leading to the activation of the immune system . It also recognizes other pathogen-associated molecular patterns (PAMPs) such as lipoteichoic acid from Gram-positive bacteria .
Mouse Anti-Human Biotin antibodies are monoclonal antibodies produced in mice that are specific to human antigens and conjugated with biotin . Biotin, also known as Vitamin B7, is a coenzyme that is widely used in molecular biology due to its strong affinity for avidin and streptavidin, which allows for easy detection and purification of biotinylated molecules .
These antibodies are commonly used in various immunoassays, including:
- Enzyme-Linked Immunosorbent Assay (ELISA): For detecting and quantifying specific antigens.
- Flow Cytometry: For analyzing the expression of cell surface and intracellular molecules.
- Western Blotting: For detecting specific proteins in a sample.
The biotinylation of antibodies enhances their utility in these assays by allowing for the use of avidin or streptavidin conjugates for detection, which can be labeled with enzymes, fluorophores, or other markers .
The combination of CD14 and Mouse Anti-Human Biotin antibodies is particularly useful in research focused on the immune response to bacterial infections. By using biotinylated antibodies specific to human CD14, researchers can:
- Detect and quantify CD14 expression: This is crucial for understanding the role of CD14 in various immune responses and diseases.
- Study the interaction between CD14 and LPS: This helps in elucidating the mechanisms of immune activation and the development of therapeutic interventions.
- Investigate the role of CD14 in different cell types: Understanding how CD14 functions in monocytes, macrophages, and other cells can provide insights into its broader role in immunity.
