CD14 Antibody
CD14, Mouse Anti-Human
Cat. No.
BT28280
Source
Synonyms
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
CD14, also known as the lipopolysaccharide (LPS) receptor, is primarily found on the surface of monocytes and macrophages. Neutrophils exhibit lower levels of CD14 expression. This protein is attached to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. CD14 functions as a high-affinity receptor for complexes formed by LPS and LPS binding protein (LBP). In its soluble form, CD14 can act as an agonist for LPS at physiological concentrations, while at higher concentrations, it exhibits an antagonistic effect on LPS-induced cell activation. Furthermore, CD14 has been observed to interact with apoptotic cells.
Formulation
The antibody is provided as a lyophilized powder. Upon reconstitution, the concentration will be 1 mg/ml in a phosphate-buffered saline (PBS) solution.
Storage Procedures
The lyophilized antibody should be stored at 4°C. After reconstitution, it is recommended to use the antibody within one month. If longer storage is required, aliquoting and storage at -20°C is advised.
Solubility
To reconstitute the antibody, add deionized water (H2O) to the vial. Mix gently by inverting or swirling, ensuring the solution makes contact with the sides of the vial. Allow 30-60 seconds for the antibody to fully dissolve before use.
Applications
This antibody can be employed for blocking experiments and cell staining procedures. For staining applications, use 10 µl of antibody per 1,000,000 cells. The optimal antibody concentration for blocking LPS binding should be determined empirically by the researcher.
Available Conjugates
In addition to the unconjugated form, this antibody is also available with biotin and fluorescein isothiocyanate (FITC) conjugates. When utilizing biotin- or FITC-conjugated antibodies for staining, it is recommended to use 5-10 µl of antibody for every 1,000,000 cells.
Synonyms
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal.
Clone
hCD14.
Immunogen
Purified human B Cells
Ig SubclassMouse IgG1.
Product Science Overview
Functional Role of CD14
CD14 is a pattern recognition receptor that plays a pivotal role in the innate immune system. It recognizes and binds to LPS, a component of the outer membrane of Gram-negative bacteria, facilitating the activation of immune responses . CD14 is also involved in the recognition of other microbial components, making it a critical player in the body’s defense against infections .
Applications in Research
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