CD14 Antibody, FITC
Description
Product Specs
Product Science Overview
CD14 is a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored single-chain glycoprotein. It is primarily expressed on the surface of monocytes and macrophages, and to a lesser extent on granulocytes. CD14 functions as a high-affinity receptor for lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria, playing a crucial role in the innate immune response.
CD14 is a member of the leucine-rich repeat (LRR) protein family, characterized by the presence of multiple LRR motifs. These motifs are involved in protein-protein interactions, which are essential for the receptor’s ability to bind LPS. CD14 exists in two forms: a membrane-bound form (mCD14) and a soluble form (sCD14). The membrane-bound form is anchored to the cell surface via a GPI anchor, while the soluble form is found in the serum and other body fluids.
The primary function of CD14 is to recognize and bind LPS, facilitating the activation of the Toll-like receptor 4 (TLR4) signaling pathway. This interaction triggers a cascade of events leading to the production of pro-inflammatory cytokines and the upregulation of adhesion molecules, which are essential for the immune response to bacterial infections.
The Mouse Anti-Human CD14 antibody is a monoclonal antibody that specifically binds to the human CD14 protein. This antibody is commonly used in research and clinical applications to study the expression and function of CD14 on various cell types. The antibody is typically conjugated to a fluorescent dye, such as fluorescein isothiocyanate (FITC), to enable the detection of CD14-expressing cells by flow cytometry or immunofluorescence microscopy.
Fluorescein isothiocyanate (FITC) is a widely used fluorescent dye that emits green fluorescence upon excitation with blue light. The conjugation of FITC to the Mouse Anti-Human CD14 antibody allows for the visualization and quantification of CD14-expressing cells. The FITC-conjugated antibody can be used in various applications, including flow cytometry, immunofluorescence microscopy, and other fluorescence-based assays.
The FITC Mouse Anti-Human CD14 antibody is used in a variety of research and clinical applications, including:
- Flow Cytometry: The antibody is used to stain human peripheral blood monocytes and other CD14-expressing cells, allowing for their identification and quantification by flow cytometry.
- Immunofluorescence Microscopy: The antibody can be used to visualize CD14-expressing cells in tissue sections or cell cultures.
- Functional Assays: The antibody can be used to block CD14-mediated signaling pathways, providing insights into the role of CD14 in immune responses.
