CD14 Mouse, Sf9

CD14 is a 53-55 kDa protein expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations, such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells, also express CD14 . The protein is anchored to the cell membrane via a GPI linkage, which facilitates its role as a high-affinity receptor for LPS .

Recombinant CD14 (Mouse, Sf9) is produced in Sf9 Baculovirus cells. This recombinant protein is a single, glycosylated polypeptide chain containing 357 amino acids (16-366 a.a.) and has a molecular mass of approximately 38.3 kDa . The protein is expressed with a 6-amino acid His tag at the C-terminus, which aids in its purification through chromatographic techniques .

CD14 is involved in the clearance of gram-negative pathogens and the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils . It functions as a high-affinity receptor for complexes of LPS and LPS-binding protein (LBP). Soluble CD14, which also binds to LPS, acts as an LPS agonist at physiological concentrations and as an LPS antagonist at higher concentrations, modulating cell activation .

Recombinant CD14 (Mouse, Sf9) is used extensively in research to study the immune response to bacterial infections, the role of CD14 in various cell types, and the mechanisms of LPS recognition and signaling. It is also used in the development of therapeutic strategies targeting bacterial infections and inflammatory diseases .

The recombinant CD14 protein is typically stored at 4°C for short-term use and at -20°C for long-term storage. It is recommended to avoid multiple freeze-thaw cycles to maintain the protein’s stability and functionality .