CCL28 Rat

Mucosae-Associated Epithelial Chemokine (CCL28) Rat Recombinant
Cat. No.
BT19403
Source
Escherichia Coli.
Synonyms
MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MEC Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13.1kDa.
The Rat MEC is purified by proprietary chromatographic techniques.

Product Specs

Introduction
CCL28, a member of the CC chemokine subfamily, exhibits chemotactic activity for resting CD4+ and CD8+ T cells and eosinophils by binding to CCR3 and CCR10 chemokine receptors. It plays a crucial role in the physiology of extracutaneous epithelial tissues, including various mucosal organs, and contributes to mucosal immunity during HIV exposure and infection. Furthermore, CCL28 is implicated in the pathogenesis of inflammatory skin diseases. Notably, human CCL28 cDNA encodes a 127-amino acid precursor protein that undergoes cleavage of a 22-amino acid signal peptide to yield the mature 105-amino acid protein. High conservation exists between human and mouse CCL28, with 83% amino acid identity in their mature regions. CCL28 exhibits the highest homology with CCL27/CTACK. Expression analysis revealed the highest levels of human and mouse CCL28 RNA in both normal and pathological colon tissues, with epithelial cells being the primary source of CCL28 protein. Human CCL28 RNA was also detected in normal and asthmatic lung tissues.
Description
Recombinant MEC Rat, produced in E. coli, is a non-glycosylated polypeptide chain comprising 116 amino acids, with a molecular weight of 13.1 kDa. The purification of Rat MEC is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a 0.2 µm filtered solution concentrated to 20mM PB, at a pH of 7.4, and 150mM NaCl.
Solubility
To reconstitute the lyophilized MEC, it is recommended to dissolve it in sterile 18 MΩ cm H₂O at a concentration not less than 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized MEC remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it desiccated below -18°C. After reconstitution, MEC should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to store it below -18°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
Purity greater than 95.0% as determined by: (a) RP-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
The biological activity is determined based on the protein's chemoattractant properties towards murine lymphocytes within a concentration range of 1.0-10.0 ng/ml.
Synonyms
MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.
Source
Escherichia Coli.
Amino Acid Sequence
SEAILPIASS CCTEVSHHIP RRLLERVNSC SIQRADGDCD LAAVILHVKR RRICVSPHNP TLKRWMSASE MKNGKENLCP RKKQDSGKDR KGHTPRKHGK HGTRRIHGTH DHEAPR.

Product Science Overview

Introduction

Mucosae-Associated Epithelial Chemokine (CCL28), also known as mucosae-associated epithelial chemokine (MEC), is a member of the CC chemokine family. This chemokine is predominantly expressed in epithelial tissues of various mucosal sites, including the large and small intestines, bronchi, mammary glands, and salivary glands . CCL28 plays a crucial role in mucosal immunity by attracting IgA-secreting plasma cells (IgA-ASC) to mucosal surfaces .

Structure and Function

CCL28 is a small, cationic polypeptide with two to three intramolecular disulfide bonds . It signals via the chemokine receptors CCR3 and CCR10, which are expressed on IgA-secreting plasma cells . The chemokine is known for its dual roles in mucosal immunity: as a chemoattractant for cells expressing CCR10 and/or CCR3 and as a broad-spectrum antimicrobial protein .

Expression and Regulation

CCL28 is selectively expressed in certain mucosal tissues such as exocrine glands, trachea, and colon . It is highly expressed in salivary glands, where it is secreted into saliva and milk . The expression of CCL28 is regulated by various factors, including microbial infections and inflammatory cytokines .

Role in Mucosal Immunity

CCL28 plays a pivotal role in mucosal immunity by recruiting IgA-secreting plasma cells to mucosal surfaces . These plasma cells produce IgA antibodies, which are essential for the immune defense of mucosal surfaces. CCL28 also exhibits antimicrobial activity against a broad spectrum of pathogens, including Candida albicans, Gram-negative bacteria, and Gram-positive bacteria .

Applications in Research and Medicine

The recombinant form of CCL28 (Rat Recombinant) is used in various research applications to study its role in mucosal immunity and its potential therapeutic applications . Research has shown that CCL28 can modulate immunity in HIV infection by recruiting IgA-secreting plasma cells to mucosal surfaces . This makes CCL28 a potential candidate for inclusion in mucosal vaccines to prevent infections .

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