CCL28 Mouse

Mucosae-Associated Epithelial Chemokine Mouse Recombinant (CCL28)
Cat. No.
BT19175
Source
Escherichia Coli.
Synonyms
MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

CCL28 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12.6 kDa.
The CCL28 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
CCL28, a member of the CC chemokine subfamily, exhibits chemotactic properties towards resting CD4 and CD8 T cells, as well as eosinophils. This chemokine interacts with chemokine receptors CCR3 and CCR10 and plays a role in the physiological functions of extracutaneous epithelial tissues, including various mucosal organs. CCL28 contributes to mucosal immunity during HIV exposure and infection and is implicated in the pathogenesis of inflammatory skin diseases. Notably, human CCL28 cDNA encodes a precursor protein of 127 amino acids, including a putative signal peptide of 22 amino acids. Cleavage of this signal peptide yields the mature protein comprising 105 amino acids. Human and mouse CCL28 share a high degree of conservation, with 83% amino acid identity in their mature forms. CCL28 exhibits the highest homology with CCL27/CTACK. Studies have revealed that both human and mouse CCL28 RNA expression is most abundant in normal and diseased colon tissues, with epithelial cells being the primary source of CCL28 protein. Additionally, human CCL28 RNA has been detected in both normal and asthmatic lung tissues.
Description
Recombinant CCL28 Mouse, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 111 amino acids. It has a molecular weight of 12.6 kDa. The purification process of CCL28 involves proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 1 mg/ml solution in 20 mM Phosphate buffer (pH 7.4) containing 150 mM NaCl.
Solubility
To reconstitute lyophilized CCL28, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized CCL28 is stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at -18°C. After reconstitution, CCL28 should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to store it at -18°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity of CCL28 is determined using the following methods and exceeds 97.0%:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis
Biological Activity
The biological activity of CCL28 is assessed by its ability to induce chemotaxis in mouse lymphocytes. This is measured using a concentration range of 1-10 ng/ml, which corresponds to a specific activity of 100,000-1,000,000 IU/mg.
Synonyms
MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.
Source
Escherichia Coli.
Amino Acid Sequence
SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R.

Product Science Overview

Introduction

Mucosae-Associated Epithelial Chemokine (CCL28), also known as MEC, is a CC chemokine that plays a crucial role in mucosal immunity. It is known to bind to chemokine receptors CCR3 and CCR10, facilitating the recruitment of IgA-secreting plasma cells (IgA-ASC) to mucosal tissues . This chemokine is particularly significant in the context of mucosal immunity, where it helps in the defense against pathogens at mucosal surfaces.

Expression and Function

CCL28 is selectively expressed in various mucosal tissues, including exocrine glands, trachea, and colon . It is also found in the salivary glands, where it is expressed at high levels . The primary function of CCL28 is to chemoattract IgA-ASC to mucosal sites, such as the large and small intestines, bronchi, mammary glands, and salivary glands . This recruitment is essential for the production of IgA antibodies, which play a critical role in mucosal immunity by neutralizing pathogens and preventing their entry into the body.

Role in HIV Infection

Research has shown that CCL28 modulates immunity in the context of HIV infection. It has been observed that CCL28 levels are augmented in the breast milk, plasma, and saliva of HIV-infected and exposed individuals . This increase in CCL28 is associated with a higher concentration of CCR3+ and CCR10+ B lymphocytes in these individuals . Additionally, CCL28 concentration in breast milk has been linked to longer survival in HIV vertically-infected children . These findings suggest that CCL28 plays a significant role in mediating mucosal immunity in HIV exposure and infection.

Potential in Vaccine Development

Given its role in mucosal immunity, CCL28 has been considered for inclusion in mucosal vaccines to prevent infections, such as HIV, by modulating IgA-ASC . The administration of CCL28 has been shown to significantly increase gastrointestinal mucosal IgA-ASC in immunized mice . This indicates that CCL28 could be a valuable component in the development of vaccines aimed at enhancing mucosal immunity.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CCL28 Human
Mucosae-Associated Epithelial Chemokine Human Recombinant (CCL28)
Cat. No.
BT18983
Source
Escherichia Coli.
CCL28 Human, His
Mucosae-Associated Epithelial Chemokine Human Recombinant (CCL28), His Tag
Cat. No.
BT19064
Source
Escherichia Coli.
CCL28 Rat
Mucosae-Associated Epithelial Chemokine (CCL28) Rat Recombinant
Cat. No.
BT19403
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.