CCL28 Human, His

Mucosae-Associated Epithelial Chemokine Human Recombinant (CCL28), His Tag
Cat. No.
BT19064
Source
Escherichia Coli.
Synonyms
MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CCL28 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 126 amino acids (23-127 a.a.) and having a molecular mass of 14.3 kDa.
The CCL28 is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CCL28, a member of the CC chemokine subfamily, exhibits chemotactic activity towards resting CD4 and CD8 T cells and eosinophils. It binds to chemokine receptors CCR3 and CCR10, playing a role in the physiological functions of extracutaneous epithelial tissues, including various mucosal organs. CCL28 contributes to mucosal immunity in the context of HIV exposure and infection. Additionally, it is implicated in the pathogenesis of inflammatory skin diseases. Human CCL28 cDNA encodes a precursor protein of 127 amino acids, including a putative 22 amino acid signal peptide that is cleaved to generate the mature 105 amino acid protein. Notably, human and mouse CCL28 share significant homology, with 83% amino acid identity in their mature regions. Among CC chemokines, CCL28 exhibits the highest homology with CCL27/CTACK. Expression analysis revealed that human and mouse CCL28 RNA expression is most abundant in both normal and diseased colon tissue, with epithelial cells being the primary source of CCL28 protein. Human CCL28 RNA is also detectable in normal and asthmatic lung tissues.
Description
Recombinant Human CCL28, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 126 amino acids (23-127 a.a.). It has a molecular weight of 14.3 kDa. A 20 amino acid His-Tag is fused to the N-terminus of CCL28, and the protein is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CCL28 protein is supplied in a solution containing 10mM Sodium Citrate (pH 3.5) and 10% Glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, it is advisable to add a carrier protein such as HSA or BSA (0.1%). Repeated freezing and thawing of the product should be avoided.
Purity
The purity of CCL28 is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MILPIASSCC TEVSHHISRR LLERVNMCRI QRADGDCDLA AVILHVKRRR ICVSPHNHTV KQWMKVQAAK KNGKGNVCHR KKHHGKRNSN RAHQGKHETY GHKTPY.

Product Science Overview

Introduction

Mucosae-Associated Epithelial Chemokine (CCL28), also known as MEC, is a chemokine that plays a crucial role in the immune system. It is involved in the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10 . CCL28 is produced by columnar epithelial cells within various tissues, including the gut, lung, breast, and salivary glands .

Function and Mechanism

CCL28 is known for its ability to recruit IgA-secreting plasma cells (IgA-ASC) to the mucosal lamina propria (MLP) . This recruitment is essential for maintaining mucosal immunity, as IgA is a critical antibody in mucosal surfaces. CCL28 binds to CCR3 and CCR10, which are expressed on IgA-ASC, facilitating their migration to mucosal sites .

Role in HIV Infection

Research has shown that CCL28 modulates immunity in HIV infection. It has been observed that CCL28 levels are augmented in the breast milk, plasma, and saliva of HIV-infected and exposed individuals . This increase in CCL28 is associated with longer survival in HIV vertically-infected children and a significant increase in gastrointestinal mucosal IgA-ASC in VSV-immunized mice receiving CCL28 .

Applications in Vaccine Development

Given its role in mucosal immunity, CCL28 is being considered for inclusion in mucosal vaccines to prevent infections such as HIV. By modulating IgA-ASC, CCL28 can enhance the immune response at mucosal surfaces, providing a potential strategy for vaccine development .

Human Recombinant CCL28 (His Tag)

The human recombinant form of CCL28, tagged with a His (histidine) tag, is used in research to study its function and potential therapeutic applications. The His tag allows for easy purification and detection of the recombinant protein, facilitating various experimental procedures.

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