APOH Human, sf9

Apolipoprotein-H (APOH), also known as Beta-2-glycoprotein 1 (B2GPI), is a glycoprotein that plays a crucial role in the human body. It is synthesized by liver cells and is present in the blood, associated with plasma lipoproteins . APOH is involved in various physiological processes, including the inhibition of the intrinsic blood coagulation cascade and the binding of certain antiphospholipid antibodies (APA) to anionic phospholipids .

The human recombinant Apolipoprotein-H produced in Sf9 insect cells is a glycosylated polypeptide chain containing 326 amino acids, with a molecular mass of 38,200 Daltons (excluding glycosylation) and a total mass of approximately 45 kDa . The recombinant protein is expressed with a 6xHis tag and purified using proprietary chromatographic techniques .

APOH attaches to various negatively charged substances such as heparin, phospholipids, and dextran sulfate . By binding to phospholipids on the surface of damaged cells, APOH prevents the activation of the intrinsic blood coagulation cascade . This function is essential in maintaining the balance of blood coagulation and preventing excessive clot formation.

Additionally, APOH is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipids . This binding is significant in the context of autoimmune disorders, where APA can target phospholipids and lead to complications such as thrombosis.

The recombinant Apolipoprotein-H (Human, Sf9) is primarily used for laboratory research purposes . It is supplied in a sterile filtered clear solution and formulated in 16mM HEPES buffer pH-7.2, 200mM NaCl, and 20% glycerol . The protein is stable when stored at 4°C for short-term use and at -20°C for long-term storage .