APOH Human
Apolipoprotein-H Human
Cat. No.
BT12141
Source
Human Plasma.
Synonyms
Beta-2-glycoprotein 1, Beta-2-glycoprotein I, Apolipoprotein H, Apo-H, B2GPI, Beta(2)GPI, Activated protein C-binding protein, APC inhibitor, Anticardiolipin cofactor, APOH, B2G1, BG, B2GP-1, B2 Glycoprotein-I.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0%.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Human APOH produced in Human Plasma having a molecular mass of 50kDa and pI of 5.6-6.4. It’s a major phospholipid binding protein and an important component to measure in the assessment of anti-phospholipid syndrome. APOH is also more specific than anti-cardiolipin antibodies and its presence correlates better with thrombotic risk.
Product Specs
Introduction
Apolipoprotein H (APOH), also known as Beta-2-glycoprotein I, is a multifunctional protein with a high affinity for negatively charged substances such as heparin, phospholipids, and dextran sulfate. One of its key roles is inhibiting the intrinsic pathway of blood coagulation by binding to phospholipids exposed on damaged cell surfaces. APOH is primarily synthesized in the liver and secreted into the bloodstream.
Description
This product consists of human APOH purified from human plasma. It has a molecular weight of 50 kDa and an isoelectric point (pI) ranging from 5.6 to 6.4. As a prominent phospholipid-binding protein, APOH serves as a crucial marker in diagnosing antiphospholipid syndrome (APS). Compared to anti-cardiolipin antibodies, APOH demonstrates superior specificity, and its presence exhibits a stronger correlation with thrombotic events.
Physical Appearance
This product is a sterile, filtered, white powder that has been lyophilized (freeze-dried).
Formulation
The lyophilization process was carried out using a 0.02M ammonium bicarbonate (NH4HCO3) solution.
Solubility
To reconstitute the lyophilized APOH powder, it is recommended to use a phosphate buffer solution with a pH greater than 7.0 and containing 0.15M sodium chloride (NaCl).
Stability
While human APOH exhibits stability at room temperature for a period of 3 weeks, it is advisable to store the product between 2-8°C to maintain optimal stability.
Purity
The purity of this product is guaranteed to be greater than 96.0%.
Human Virus Test
The starting material used for production has undergone rigorous testing and has been confirmed negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
Synonyms
Beta-2-glycoprotein 1, Beta-2-glycoprotein I, Apolipoprotein H, Apo-H, B2GPI, Beta(2)GPI, Activated protein C-binding protein, APC inhibitor, Anticardiolipin cofactor, APOH, B2G1, BG, B2GP-1, B2 Glycoprotein-I.
Source
Human Plasma.
Product Science Overview
Structure and Function
Apolipoprotein-H is primarily involved in the binding of cardiolipin, a phospholipid found in the inner mitochondrial membrane . When Apo-H binds to cardiolipin, both molecules undergo substantial structural changes . This interaction is crucial for the protein’s role in the intrinsic pathway of blood coagulation .
The protein contains a stretch of positively charged amino acids (positions 282-287) that are essential for its phospholipid-binding activity . These amino acids include Lysine, Asparagine, Glutamic acid, and Lysine .
Biological Processes
Apolipoprotein-H is involved in several biological processes, including:
- Blood Coagulation: Apo-H plays a role in both the positive and negative regulation of blood coagulation . It is involved in the intrinsic pathway of blood coagulation and the regulation of fibrinolysis .
- Lipid Metabolism: Apo-H is a significant component in the transport and metabolism of triglycerides . It positively regulates lipoprotein lipase activity, which is essential for the breakdown of triglycerides .
- Immune Response: Apo-H has been implicated in the body’s immune response, particularly in the negative regulation of endothelial cell migration and proliferation .
Clinical Significance
Apolipoprotein-H is a critical marker in the assessment of anti-phospholipid syndrome (APS), an autoimmune disorder characterized by the presence of anti-phospholipid antibodies . APS is associated with an increased risk of thrombosis, and the presence of Apo-H correlates better with thrombotic risk than anti-cardiolipin antibodies .
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