APOH

Apolipoprotein-H Human Recombinant
Cat. No.
BT11767
Source
Escherichia Coli.
Synonyms
Beta-2-glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Apolipoprotein H, Apo-H, Beta-2-glycoprotein I, B2GPI, Beta(2)GPI, APOH, B2G1, BG, B2GP1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

APOH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 349 amino acids (20-345 a.a.) and having a molecular mass of 38.6kDa.
APOH is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Description
Recombinant human APOH protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 349 amino acids, with a sequence spanning from amino acid 20 to 345, resulting in a molecular weight of 38.6 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus to facilitate purification via proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The APOH protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 2 M urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the APOH protein solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. Repeated freezing and thawing of the protein solution should be avoided.
Purity
The purity of the APOH protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Beta-2-glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Apolipoprotein H, Apo-H, Beta-2-glycoprotein I, B2GPI, Beta(2)GPI, APOH, B2G1, BG, B2GP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSGRTCPKP DDLPFSTVVP LKTFYEPGEE ITYSCKPGYV SRGGMRKFIC PLTGLWPINT LKCTPRVCPF AGILENGAVR YTTFEYPNTI SFSCNTGFYL NGADSAKCTE EGKWSPELPV CAPIICPPPS IPTFATLRVY KPSAGNNSLY RDTAVFECLP QHAMFGNDTI TCTTHGNWTK LPECREVKCP FPSRPDNGFV NYPAKPTLYY KDKATFGCHD GYSLDGPEEI ECTKLGNWSA MPSCKASCKV PVKKATVVYQ GERVKIQEKF KNGMLHGDKV SFFCKNKEKK CSYTEDAQCI DGTIEVPKCF KEHSSLAFWK TDASDVKPC.

Product Science Overview

Introduction

Apolipoprotein-H (Apo-H), also known as β2-glycoprotein I, is a multifunctional plasma protein encoded by the APOH gene in humans . This protein plays a crucial role in lipid metabolism and thrombotic regulation, making it a significant subject of study in cardiovascular health .

Structure and Function

Apo-H is a 38 kDa protein that consists of four tandem Sushi/SCR repeats followed by one Sushi-like repeat . It is variably glycosylated and belongs to the complement control superfamily of proteins . One of its primary functions is to bind cardiolipin, a phospholipid found in the inner mitochondrial membrane . When bound, both cardiolipin and Apo-H undergo significant structural changes .

Physiological Roles

Apo-H is involved in various physiological processes, including:

  • Lipid Metabolism: Apo-H interacts with lipoproteins, aiding in the transport and metabolism of lipids .
  • Thrombotic Regulation: It plays a role in the regulation of blood coagulation, acting as an inhibitor of the intrinsic coagulation pathway .
  • Immune Response: Apo-H has been implicated in the modulation of the immune response, particularly in the context of autoimmune diseases .
Genetic Implications

Mutations in the APOH gene can lead to alterations in the structure and function of Apo-H, potentially contributing to various cardiovascular and autoimmune diseases . Understanding these genetic implications is crucial for developing targeted therapies.

Recombinant Production

Human recombinant Apo-H is produced using Escherichia coli (E. coli) as the expression system . The recombinant protein is a single, non-glycosylated polypeptide chain containing 349 amino acids and has a molecular mass of 38.6 kDa . It is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .

Applications

Recombinant Apo-H has several potential applications:

  • Therapeutic Target: Due to its role in lipid metabolism and thrombotic regulation, Apo-H is being explored as a potential therapeutic target for cardiovascular diseases .
  • Research Tool: It is used in various research applications to study its physiological roles and genetic implications .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

APOH Bovine
Apoliporotein-H Bovine
Cat. No.
BT12044
Source
Fetal calf serum (FCS).
APOH Human
Apolipoprotein-H Human
Cat. No.
BT12141
Source
Human Plasma.
APOH Human, sf9
Apolipoprotein-H Human Recombinant, Sf9
Cat. No.
BT12207
Source
Sf9 insect cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.