ANXA4 Human

Annexin A4 Human Recombinant
Cat. No.
BT20438
Source
Escherichia Coli.
Synonyms
ANX4, ANXA4, Annexin-IV, Lipocortin-4, Lipocortin-IV, Annexin-4, Annexin A4, Endonexin I, Chromobindin-4, Protein II, P32.5, Placental anticoagulant protein II, PAP-II, PP4-X, 35-beta calcimedin, Carbohydrate-binding protein p33/p41, PIG28, ZAP36, MGC75105, DKFZp686H02120.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa. ANXA4 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Annexin A4 (ANXA4) belongs to the annexin family, a group of calcium-dependent phospholipid binding proteins. ANXA4 exhibits a 45%-59% sequence identity with other family members and shares similar size and exon-intron organization. It interacts with ATP and displays in vitro anticoagulant activity by inhibiting phospholipase A2 activity. ANXA4 expression is restricted to epithelial cells. As a Calcium/phospholipid-binding protein, ANXA4 facilitates membrane fusion and participates in exocytosis.
Description
Recombinant human ANXA4, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing 321 amino acids (residues 1-321). The protein has a molecular weight of 36 kDa and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The ANXA4 protein solution is supplied in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol and 0.2M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of ANXA4 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
ANX4, ANXA4, Annexin-IV, Lipocortin-4, Lipocortin-IV, Annexin-4, Annexin A4, Endonexin I, Chromobindin-4, Protein II, P32.5, Placental anticoagulant protein II, PAP-II, PP4-X, 35-beta calcimedin, Carbohydrate-binding protein p33/p41, PIG28, ZAP36, MGC75105, DKFZp686H02120.
Source
Escherichia Coli.
Amino Acid Sequence
MAMATKGGTV KAASGFNAME DAQTLRKAMK GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ VIVGMMTPTV LYDVQELRRA MKGAGTDEGC LIEILASRTP EEIRRISQTY QQQYGRSLED DIRSDTSFMF QRVLVSLSAG GRDEGNYLDD ALVRQDAQDL YEAGEKKWGT DEVKFLTVLC SRNRNHLLHV FDEYKRISQK DIEQSIKSET SGSFEDALLA IVKCMRNKSA YFAEKLYKSM KGLGTDDNTL IRVMVSRAEI DMLDIRAHFK RLYGKSLYSF IKGDTSGDYR KVLLVLCGGD D.

Product Science Overview

Gene and Protein Structure

The ANXA4 gene is located on chromosome 2 in humans and encodes a protein that shares 45 to 59% identity with other members of the annexin family . The protein structure of Annexin A4 is characterized by its ability to bind to phospholipids in a calcium-dependent manner. This binding is crucial for its role in cellular processes.

Expression and Localization

Annexin A4 is almost exclusively expressed in epithelial cells . It has been isolated from human placenta and is known to interact with ATP. The protein is also found in various cellular components, including the cytoplasm, nuclear membrane, vesicle membrane, plasma membrane, and extracellular exosomes .

Functions and Activities

Annexin A4 has several important functions:

  • Phospholipase Inhibition: It inhibits phospholipase A2 activity, which is involved in the hydrolysis of phospholipids .
  • Anticoagulant Activity: In vitro studies have shown that Annexin A4 possesses anticoagulant properties .
  • Calcium Ion Binding: The protein binds calcium ions, which is essential for its interaction with phospholipids .
  • Signal Transduction: Annexin A4 is involved in intracellular signal transduction pathways, contributing to the regulation of cellular growth and differentiation .
Biological Processes

Annexin A4 plays a role in various biological processes, including:

  • Epithelial Cell Differentiation: It is involved in the differentiation of epithelial cells .
  • Regulation of Transcription: The protein negatively regulates transcription by RNA polymerase II .
  • Apoptosis: Annexin A4 negatively regulates the apoptotic process, thereby contributing to cell survival .
Clinical Relevance

The unique properties of Annexin A4 make it a subject of interest in clinical research. Its anticoagulant activity and role in phospholipase inhibition suggest potential therapeutic applications. Additionally, its involvement in epithelial cell differentiation and apoptosis regulation highlights its importance in various physiological and pathological conditions.

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