ANXA1 Human
Annexin A1 Human Recombinant
Cat. No.
BT19749
Source
Escherichia Coli.
Synonyms
ANX1, LPC1, ANXA1, Lipocortin I, Calpactin II, Chrombindin-9, p35, Annexin-1, Phospholipase A2 inhibitory protein, Annexin I, Annexin A1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ANXA1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 346 amino acids (1-346 a.a.) and having a molecular mass of 38.7 kDa.
ANXA1 is purified by proprietary chromatographic techniques.
ANXA1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Annexin A1 (ANXA1) is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. These proteins, with molecular weights ranging from 35 kDa to 40 kDa, are found on the cytoplasmic side of the plasma membrane. ANXA1, with a molecular weight of 40 kDa, exhibits phospholipase A2 inhibitory activity and binds two to four calcium ions with high affinity. Given that phospholipase A2 is crucial for the production of inflammatory mediators like prostaglandins and leukotrienes, ANXA1 is thought to possess anti-inflammatory properties. Additionally, ANXA1 facilitates membrane fusion and plays a role in exocytosis. The specific recognition of ANXA1 by immunocytochemistry offers a sensitive and reliable diagnostic tool for hairy cell leukemia.
Description
This product consists of the human ANXA1 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 346 amino acids (amino acids 1-346) and has a molecular weight of 38.7 kDa. The protein is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The ANXA1 protein is provided in a solution containing 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 1 mM DTT (dithiothreitol), and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein such as albumin (0.1% HSA or BSA) is advisable. Repeated freezing and thawing should be avoided.
Purity
The purity of this protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
ANX1, LPC1, ANXA1, Lipocortin I, Calpactin II, Chrombindin-9, p35, Annexin-1, Phospholipase A2 inhibitory protein, Annexin I, Annexin A1.
Source
Escherichia Coli.
Amino Acid Sequence
MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK
PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL
LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKCATSKPAFFAE KLHQAMKGVG TRHKALIRIM VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN.
PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL
LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKCATSKPAFFAE KLHQAMKGVG TRHKALIRIM VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN.
Product Science Overview
Introduction
Annexin A1, also known as Lipocortin 1, Calpactin II, Lipomodulin, and Chromobindin 9, is a member of the annexin family of proteins. These proteins are characterized by their ability to bind to phospholipids in a calcium-dependent manner. Annexin A1 (ANXA1) plays a crucial role in various cellular processes, including membrane dynamics and the regulation of membrane-associated proteins .
Structure and Properties
Human Annexin A1 consists of four annexin repeats, each comprising 60-70 amino acids. These repeats assemble into a curved disc-like domain that binds to membrane phospholipids. The recombinant form of Annexin A1 is typically produced in E. coli and includes a C-terminal 6-His tag for purification purposes. The protein has a predicted molecular mass of 39.4 kDa and appears as a 38 kDa band under reducing conditions in SDS-PAGE .
Biological Functions
Annexin A1 is involved in a variety of biological functions:
- Anti-inflammatory Effects: Annexin A1 is known for its anti-inflammatory properties. It inhibits the production of pro-inflammatory mediators and promotes the resolution of inflammation.
- Cell Proliferation and Apoptosis: The protein can inhibit the proliferation of certain cancer cells, such as A549 human lung carcinoma cells. It also plays a role in the regulation of apoptosis.
- Immune Response: Annexin A1 is released from activated neutrophils and can bind to G protein-coupled receptors like FPR2/ALXR and FPR1, mediating cellular effects via autocrine and paracrine pathways .
Expression and Tissue Distribution
Recombinant Production and Applications
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