ANXA2
Annexin A2 Human Recombinant
Cat. No.
BT20150
Source
Escherichia Coli.
Synonyms
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, ANXA2, Annexin A2, Annexin-2, Annexin II, Lipocortin II, Calpactin-1 heavy chain, Calpactin I heavy chain, Chromobindin-8, p36, Protein I, Placental anticoagulant protein IV.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ANXA2 Human Recombinant produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 376 amino acids (1-339a.a.) and having a molecular mass of 42.8 kDa. ANXA2 is fused to a 37 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
ANXA2, a member of the annexin family, plays a crucial role in regulating cellular growth and signal transduction pathways. This protein acts as an autocrine factor that stimulates osteoclast formation and bone resorption, making it significant in conditions like sickle cell osteonecrosis. Reduced ANXA2 expression is linked to osteosarcoma metastases. Functioning as part of the cell surface vitamin D binding protein binding site complex, ANXA2 mediates the chemotactic cofactor effect. Its involvement extends to dysferlin deficiency and muscular dystrophies. Notably, an up-regulation of ANXA2 is observed during human colon adenocarcinoma cell differentiation.
Description
Recombinant human ANXA2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 376 amino acids (1-339a.a.). With a molecular mass of 42.8 kDa, this protein is fused to a 37 amino acid His-Tag at its N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The ANXA2 solution (0.5mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8), 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It is crucial to avoid repeated freeze-thaw cycles.
Purity
The purity of ANXA2 is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, ANXA2, Annexin A2, Annexin-2, Annexin II, Lipocortin II, Calpactin-1 heavy chain, Calpactin I heavy chain, Chromobindin-8, p36, Protein I, Placental anticoagulant protein IV.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMST VHEILCKLSL EGDHSTPPSA YGSVKAYTNF DAERDALNIE TAIKTKGVDE VTIVNILTNR SNAQRQDIAF AYQRRTKKEL ASALKSALSG HLETVILGLL KTPAQYDASE LKASMKGLGT DEDSLIEIIC SRTNQELQEI NRVYKEMYKT DLEKDIISDT SGDFRKLMVA LAKGRRAEDG SVIDYELIDQ DARDLYDAGV KRKGTDVPKW ISIMTERSVP HLQKVFDRYK SYSPYDMLES IRKEVKGDLE NAFLNLVQCI QNKPLYFADR LYDSMKGKGT RDKVLIRIMV SRSEVDMLKI RSEFKRKYGK SLYYYIQQDT KGDYQKALLY LCGGDD.
Product Science Overview
Structure and Function
Annexin A2 is a 39 kDa protein composed of a unique N-terminal domain and a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain consists of four 60-70 amino acid annexin repeats, forming a tightly packed disc known as the annexin domain . This structure allows Annexin A2 to bind to phospholipids in a calcium-dependent manner.
The primary functions of Annexin A2 include:
- Cytoskeletal Interactions: Annexin A2 interacts with the cytoskeleton, contributing to cellular shape and motility.
- Phospholipase Inhibition: It inhibits phospholipase A2, an enzyme involved in the release of arachidonic acid, a precursor for various signaling molecules.
- Regulation of Cellular Growth: Annexin A2 plays a role in cell proliferation and differentiation.
- Signal Transduction Pathways: It is involved in intracellular signaling pathways, influencing various cellular responses .
Recombinant Human Annexin A2
Recombinant human Annexin A2 is produced using E. coli expression systems. The recombinant protein typically includes a C-terminal 6-His tag for purification purposes . It is available in both carrier-free and carrier-protein formulations, depending on the intended application. Carrier proteins like Bovine Serum Albumin (BSA) are often added to enhance protein stability and shelf-life .
Applications
Recombinant Annexin A2 is widely used in research to study its role in various physiological and pathological processes, including:
- Osteoclast Formation and Bone Resorption: Annexin A2 functions as an autocrine factor, increasing osteoclast formation and bone resorption .
- Cancer Research: Up-regulation of Annexin A2 is associated with colon adenocarcinoma cell differentiation and osteosarcoma metastases .
- Muscular Dystrophies: Annexin A2 is involved in the pathology of muscular dystrophies .
- Sickle Cell Osteonecrosis: It is associated with sickle cell osteonecrosis, a condition characterized by bone tissue death due to lack of blood supply .
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