ANXA6 Human

Annexin A6 is the largest member of the annexin family, with a molecular weight of approximately 68 kDa . It contains eight annexin repeats, which are sequences of about 70 amino acids each. These repeats are separated by linking sequences of variable lengths . The protein is encoded by the ANXA6 gene located on chromosome 5q32-q34 .

Annexin A6 is expressed in various tissues and is found both intracellularly and on the cell surface. It is particularly abundant in the plasma membrane and endosomal compartments . Additionally, it is secreted into the bile and is present on the cell surface .

Annexin A6 is involved in several critical cellular functions:

1. Calcium Signaling: It plays a role in the regulation of calcium ion conductance and the release of Ca2+ from intracellular stores .
2. Membrane Dynamics: Annexin A6 is involved in membrane vesicle trafficking, endosome aggregation, and vesicle fusion during exocytosis .
3. Signal Transduction: As a multifunctional scaffold protein, it interacts with phospholipid membranes and various signaling proteins, participating in signal transduction pathways .
4. Cholesterol Homeostasis: It is involved in the regulation of cholesterol homeostasis .
5. Membrane Repair: Annexin A6 contributes to the repair of membrane domains .

Annexin A6 has been implicated in various pathological conditions, including cancer. Its expression is consistently altered during tumor formation and progression, making it a potentially valuable marker for the diagnosis, progression, and treatment of different cancers . Additionally, it has been associated with diseases such as Kwashiorkor and Autosomal Recessive Nonsyndromic Deafness 116 .

Recombinant human Annexin A6 is produced using expression systems such as E. coli. The recombinant protein is typically purified to a high degree of purity (over 95%) and is used for research purposes . It is available in lyophilized form and can be reconstituted for various experimental applications .