ANGPTL3 (PAT23D8AT) Antibody

Mouse Anti Human Angiopoietin Like Protein 3 Clone PAT23D8AT
Cat. No.
BT3515
Source
Synonyms

Angiopoietin Like 3, Angiopoietin 5, ANGPT5, ANG-5, Angiopoietin-Related Protein 3 , Angiopoietin-Like Protein 3, Angiopoietin-Like 3, Angiopoietin-5, FHBL2, ANL3.

Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
ANGPTL3 and ANGPTL4 are angiopoietin-like proteins primarily produced by the liver. They play a crucial role in regulating triglyceride metabolism by inhibiting the breakdown of triglyceride-rich lipoproteins. Studies have shown that Angptl3 and Angptl4 differentially inhibit Lipoprotein lipase (LPL) to regulate circulating triglyceride levels during different nutritional states, such as feeding and fasting. ANGPTL3's molecular structure resembles angiopoietins (vascular endothelial growth factors). Experiments using deletion mutants of human Angiopoietin 5 have demonstrated that the N-terminal domain (fragment 17-207), rather than the C-terminal fibrinogen-like domain (fragment 207-460), is responsible for increasing plasma triglyceride levels in mice.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer containing phosphate-buffered saline (PBS) at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody can be kept at 4°C. For extended storage, it is recommended to store the antibody at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
The ANGPTL3 antibody has been validated for use in ELISA, Western blot analysis, and immunocytochemistry/immunofluorescence (ICC/IF) assays. These tests confirm the antibody's specificity and reactivity. However, it is essential to optimize the antibody concentration for each specific application to achieve the best results.
Synonyms

Angiopoietin Like 3, Angiopoietin 5, ANGPT5, ANG-5, Angiopoietin-Related Protein 3 , Angiopoietin-Like Protein 3, Angiopoietin-Like 3, Angiopoietin-5, FHBL2, ANL3.

Purification Method

ANGPTL3 antibody was purified by protein-A affinity chromatography.

Type
Mouse Anti Human Monoclonal.
Clone

PAT23D8AT.

Immunogen

Anti-human ANGPTL3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ANGPTL3 protein 243-460 amino acids purified from E. coli.

Ig Subclass

Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Introduction

Mouse Anti-Human Angiopoietin-Like Protein 3 (ANGPTL3) Clone PAT23D8AT is a monoclonal antibody specifically designed to target and bind to human ANGPTL3. This antibody is produced in mice and is widely used in various research applications, including Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunocytochemistry (ICC), and Immunofluorescence (IF) .

Angiopoietin-Like Protein 3 (ANGPTL3)

ANGPTL3 is a member of the angiopoietin-like protein family, which plays a crucial role in lipid metabolism and angiogenesis. It is primarily expressed in the liver and has been shown to regulate plasma lipid levels by inhibiting lipoprotein lipase (LPL) activity. Elevated levels of ANGPTL3 are associated with hyperlipidemia and an increased risk of cardiovascular diseases .

Production and Cloning

The PAT23D8AT clone is derived from a single B-cell clone of a mouse immunized with human ANGPTL3. The monoclonal antibody is produced using hybridoma technology, where the B-cells are fused with myeloma cells to create a hybrid cell line capable of producing large quantities of the antibody. This process ensures the specificity and consistency of the antibody .

Applications
  1. Western Blot (WB): The antibody is used to detect ANGPTL3 in tissue lysates and cell extracts. It binds specifically to the target protein, allowing for its visualization and quantification.
  2. Enzyme-Linked Immunosorbent Assay (ELISA): The antibody is used as a capture or detection antibody in ELISA assays to measure the concentration of ANGPTL3 in biological samples.
  3. Immunocytochemistry (ICC): The antibody is used to stain cells and visualize the localization of ANGPTL3 within the cell.
  4. Immunofluorescence (IF): The antibody is conjugated with fluorescent dyes to visualize ANGPTL3 in cells and tissues under a fluorescence microscope .

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