ANGPTL3 Human, HEK

Angiopoietin Like Protein 3 Human Recombinant, HEK
Cat. No.
BT9946
Source
HEK 293.
Synonyms
Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ANGPTL3 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 17-460) containing a total of 450 amino acids, having a molecular mass of 52.6kDa (calculated) and fused to a 6 aa His tag at C-Terminus.
The Human ANGPTL3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
ANGPTL3 and ANGPTL4, primarily produced by the liver, are angiopoietin-like proteins that play a crucial role in regulating triglyceride metabolism. They achieve this by inhibiting the breakdown of triglyceride-rich lipoproteins. Experimental evidence demonstrates that Angptl3 and Angptl4 differentially inhibit Lipoprotein lipase (LPL) to regulate circulating triglyceride levels during various nutritional states, such as feeding and fasting. ANGPTL3's molecular structure resembles angiopoietins, which are vascular endothelial growth factors. Studies using deletion mutants of human Angiopoietin 5 have shown that the N-terminal domain (amino acids 17-207), rather than the C-terminal fibrinogen-like domain (amino acids 207-460), is responsible for increasing plasma triglyceride levels in mice.
Description
Recombinant Human ANGPTL3, produced in HEK cells, is a single, glycosylated polypeptide chain encompassing amino acids 17-460. With a total of 450 amino acids, it has a calculated molecular mass of 52.6 kDa. The protein is fused to a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
White, lyophilized powder after filtration.
Formulation
The product is filtered through a 0.4 μm filter and lyophilized from a solution of 0.5 mg/ml in 0.05 M phosphate buffer (pH 7.4) containing 0.075 M NaCl.
Solubility
To reconstitute, add deionized water to achieve a working concentration of 0.5 mg/ml and allow the lyophilized pellet to dissolve completely. Note: This product is not sterile. Filter it through an appropriate sterile filter before use in cell culture.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize freeze-thaw cycles. Reconstituted protein can be stored at 4°C for a limited period.
Purity
Purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.
Source
HEK 293.
Amino Acid Sequence
SRIDQDNSSF DSLSPEPKSR FAMLDDVKIL ANGLLQLGHG LKDFVHKTKG QINDIFQKLN IFDQSFYDLS LQTSEIKEEE KELRRTTYKL QVKNEEVKNM SLELNSKLES LLEEKILLQQ KVKYLEEQLT NLIQNQPETP EHPEVTSLKT FVEKQDNSIK DLLQTVEDQY KQLNQQHSQI KEIENQLRRT SIQEPTEISL SSKPRAPRTT PFLQLNEIRN VKHDGIPAEC TTIYNRGEHT SGMYAIRPSN SQVFHVYCDV ISGSPWTLIQ HRIDGSQNFN ETWENYKYGF GRLDGEFWLG LEKIYSIVKQ SNYVLRIELE DWKDNKHYIE YSFYLGNHET NYTLHLVAIT GNVPNAIPEN KDLVFSTWDH KAKGHFNCPE GYSGGWWWHD ECGENNLNGK YNKPRAKSKP ERRRGLSWKS QNGRLYSIKS TKMLIHPTDS ESFEHHHHHH.

Product Science Overview

Structure and Function

ANGPTL3 has a characteristic structure similar to angiopoietins, consisting of:

  • A signal peptide
  • An N-terminal coiled-coil domain
  • A C-terminal fibrinogen-like domain (FBN-like domain)

The FBN-like domain in ANGPTL3 binds to alpha-5/beta-3 integrins, inducing endothelial cell adhesion and migration . This protein is known to act as a dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), thereby increasing plasma triglyceride and HDL cholesterol levels .

Biological Activity

ANGPTL3 is a secreted glycoprotein that intricately regulates lipid and glucose metabolism. It stimulates plasma triglycerides by inhibiting lipoprotein lipase (LPL) and suppressing triglyceride clearance through PCSK6 and FURIN recruitment . This regulation is independent of proteolytic cleavage and unaffected by GPIHBP1 .

Recombinant ANGPTL3 (Human, HEK)

Recombinant human ANGPTL3 protein is produced using HEK 293 cells, a human embryonic kidney cell line. This recombinant protein is often tagged for easy identification and purification. It is used in various functional studies, including biochemical assays and ELISA .

Key features of recombinant ANGPTL3 include:

  • Expression system: HEK 293 cells
  • Purity: ≥ 90% (SDS-PAGE)
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: DDDDK tag at the C-Terminus
Applications

Recombinant ANGPTL3 is used in research to study its role in lipid metabolism, angiogenesis, and its potential therapeutic applications. It is also used to investigate the molecular mechanisms linking ANGPTL3 to various metabolic disorders .

Storage and Stability

For optimal stability, recombinant ANGPTL3 should be stored in aliquots at -80°C. It is recommended to dissolve the protein in water and keep the final concentration for storage at least 10 µg/ml or higher .

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