ANGPTL3 (243-460) Human

Angiopoietin-like Protein 3 (243-460 a.a.) Human Recombinant
Cat. No.
BT9785
Source
E.coli.
Synonyms
Angiopoietin-Like 3, ANGPT5, Angiopoietin 5, FHBL2, ANG-5, Angiopoietin-Related Protein 3, Angiopoietin-Like Protein 3, Angiopoietin-5, ANL3, Angiopoietin-related protein 3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ANGPTL3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (243-460 a.a) and having a molecular mass of 27.7kDa.
ANGPTL3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ANGPTL3 and ANGPTL4, primarily produced by the liver, are angiopoietin-like proteins that play a crucial role in regulating triglyceride metabolism. They achieve this by inhibiting the breakdown of triglyceride-rich lipoproteins. Experimental evidence suggests that Angptl3 and Angptl4 differentially inhibit Lipoprotein lipase (LPL) based on nutritional status (feeding or fasting), thereby controlling circulating triglyceride levels. ANGPTL3 shares a structural resemblance to angiopoietins, which are vascular endothelial growth factors. Studies using deletion mutants of human Angiopoietin 5 have revealed that the N-terminal domain (amino acids 17-207) is responsible for increasing plasma triglyceride levels in mice, while the C-terminal fibrinogen-like domain (amino acids 207-460) does not exhibit this effect.
Description
Recombinant Human ANGPTL3, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 239 amino acids (243-460). It has a molecular weight of 27.7 kDa. The protein is purified using proprietary chromatographic techniques and is expressed with a 21 amino acid His-tag at the N-terminus.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The ANGPTL3 protein solution has a concentration of 1 mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It's important to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of ANGPTL3 is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Angiopoietin-Like 3, ANGPT5, Angiopoietin 5, FHBL2, ANG-5, Angiopoietin-Related Protein 3, Angiopoietin-Like Protein 3, Angiopoietin-5, ANL3, Angiopoietin-related protein 3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPAECTTIYN RGEHTSGMYA IRPSNSQVFH VYCDVISGSP WTLIQHRIDG SQNFNETWEN YKYGFGRLDG EFWLGLEKIY SIVKQSNYVL RIELEDWKDN KHYIEYSFYL GNHETNYTLH LVAITGNVPN AIPENKDLVF STWDHKAKGH FNCPEGYSGG WWWHDECGEN NLNGKYNKPR AKSKPERRRG LSWKSQNGRL YSIKSTKMLI HPTDSESFE.

Product Science Overview

Introduction

Angiopoietin-like Protein 3 (ANGPTL3) is a member of the angiopoietin-like family of proteins, which are known for their roles in lipid metabolism and angiogenesis. The specific fragment of ANGPTL3, spanning amino acids 243-460, is a recombinant human protein that has been studied for its various biological functions and potential therapeutic applications.

Structure and Function

ANGPTL3 is a glycoprotein that consists of several distinct domains, including a coiled-coil domain and a fibrinogen-like domain. The fragment encompassing amino acids 243-460 includes parts of these domains, which are crucial for its biological activity. ANGPTL3 is primarily expressed in the liver and plays a significant role in regulating lipid metabolism by inhibiting lipoprotein lipase (LPL), an enzyme responsible for the hydrolysis of triglycerides in lipoproteins .

Biological Activity

The recombinant human ANGPTL3 (243-460 a.a.) has been shown to inhibit lipoprotein lipase activity, which leads to increased levels of circulating triglycerides. This inhibition is crucial for understanding the protein’s role in lipid metabolism and its potential implications in metabolic disorders such as hyperlipidemia and cardiovascular diseases .

Therapeutic Potential

Given its role in lipid metabolism, ANGPTL3 has been a target for therapeutic interventions aimed at treating hyperlipidemia and related cardiovascular conditions. Inhibitors of ANGPTL3 are being developed to reduce plasma lipid levels and mitigate the risk of cardiovascular diseases. The recombinant form of ANGPTL3 (243-460 a.a.) is used in research to better understand its function and to develop potential therapeutic agents .

Research Applications

The recombinant ANGPTL3 (243-460 a.a.) is widely used in various research applications, including:

  • In vitro studies: To investigate the protein’s role in lipid metabolism and its interaction with other metabolic regulators.
  • Drug development: As a target for developing inhibitors that can modulate its activity and reduce lipid levels in the blood.
  • Biochemical assays: To study the protein’s structure, function, and interactions with other molecules .

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