ANGPTL3 (17-460) Human
Angiopoietin-like Protein 3 (17-460 a.a.) Human Recombinant
Cat. No.
BT9322
Source
Sf9,
Baculovirus cells.
Synonyms
Angiopoietin Like 3, Angiopoietin 5, ANGPT5, ANG-5,
Angiopoietin-Related Protein 3 , Angiopoietin-Like Protein 3, Angiopoietin-Like
3, Angiopoietin-5, FHBL2, ANL3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
ANGPTL3 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 453 amino acids (17-460 a.a.) and
having a molecular mass of 52.9kDa (Migrates at 25-70kDa on SDS-PAGE under
reducing conditions).
ANGPTL3 is expressed with an 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
ANGPTL3 and ANGPTL4, primarily produced by the liver, are angiopoietin-like proteins that play a crucial role in regulating triglyceride metabolism. They achieve this by inhibiting the breakdown of triglyceride-rich lipoproteins. Experimental evidence suggests that Angptl3 and Angptl4 differentially inhibit Lipoprotein lipase (LPL) to control circulating triglyceride levels during different nutritional states, such as feeding and fasting. ANGPTL3's molecular structure resembles angiopoietins, which are vascular endothelial growth factors. Studies using deletion mutants of human Angiopoietin 5 have shown that the N-terminal domain (fragment 17-207) is responsible for increasing plasma triglyceride levels in mice, while the C-terminal fibrinogen-like domain (fragment 207-460) is not.
Description
ANGPTL3, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with 453 amino acids (17-460 a.a.). It has a molecular mass of 52.9kDa and appears between 25-70kDa on SDS-PAGE under reducing conditions. This protein is expressed with a 6 amino acid His tag at its C-Terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ANGPTL3 protein solution is provided at a concentration of 0.25mg/ml and contains Buffered Saline (pH 7.4), 30% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the ANGPTL3 solution can be stored at 4°C. For longer periods, it should be stored frozen at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of ANGPTL3 is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Angiopoietin Like 3, Angiopoietin 5, ANGPT5, ANG-5,
Angiopoietin-Related Protein 3 , Angiopoietin-Like Protein 3, Angiopoietin-Like
3, Angiopoietin-5, FHBL2, ANL3.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
ADPSRIDQDN SSFDSLSPEP KSRFAMLDDV KILANGLLQL GHGLKDFVHK
TKGQINDIFQ KLNIFDQSFY DLSLQTSEIK EEEKELRRTT YKLQVKNEEV KNMSLELNSK LESLLEEKIL LQQKVKYLEE
QLTNLIQNQP ETPEHPEVTS LKTFVEKQDN SIKDLLQTVE DQYKQLNQQH SQIKEIENQL RRTSIQEPTE
ISLSSKPRAP RTTPFLQLNE IRNVKHDGIP AECTTIYNRG EHTSGMYAIR PSNSQVFHVY CDVISGSPWT LIQHRIDGSQ
NFNETWENYK YGFGRLDGEF WLGLEKIYSI VKQSNYVLRI ELEDWKDNKH YIEYSFYLGN HETNYTLHLV
AITGNVPNAI PENKDLVFST WDHKAKGHFN CPEGYSGGWW WHDECGENNL NGKYNKPRAK SKPERRRGLS WKSQNGRLYS
IKSTKMLIHP TDSESFEHHH HHH.
Product Science Overview
Structure and Domains
The human recombinant ANGPTL3 protein, specifically the 17-460 amino acid (a.a.) fragment, includes several important structural domains:
- N-terminal coiled-coil domain: This domain is involved in protein-protein interactions and is essential for the oligomerization of ANGPTL3 .
- C-terminal fibrinogen-like domain: This domain is similar to the fibrinogen-like domains found in angiopoietins and is involved in binding to receptors and other molecules .
Function and Activity
ANGPTL3 has several key functions:
- Inhibition of Lipoprotein Lipase (LPL): ANGPTL3 inhibits LPL, an enzyme that hydrolyzes triglycerides in lipoproteins into free fatty acids and glycerol. This inhibition leads to increased plasma triglyceride levels .
- Regulation of Lipid Metabolism: By inhibiting LPL, ANGPTL3 plays a significant role in regulating lipid metabolism and maintaining energy homeostasis .
- Promotion of Cell Expansion: ANGPTL3 has been shown to promote the expansion of liver mononuclear cells in vitro, indicating its potential role in liver function and regeneration .
Recombinant Production
The recombinant human ANGPTL3 protein (17-460 a.a.) is produced using baculovirus expression systems in insect cells (Spodoptera frugiperda, Sf 21). This method ensures high purity and activity of the protein . The recombinant protein is typically tagged with a C-terminal 10-His tag for easy purification and detection .
Applications
Recombinant ANGPTL3 is used in various research applications, including:
- Lipid Metabolism Studies: To investigate the role of ANGPTL3 in lipid metabolism and its potential as a therapeutic target for hyperlipidemia and cardiovascular diseases.
- Cell Culture: As a supplement in cell culture media to study its effects on cell growth and differentiation.
- Biochemical Assays: To measure its activity in inhibiting LPL and other related enzymes.
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