Zika NS1, HEK

Zika NS1 Protein Recombinant, HEK
Cat. No.
BT11367
Source
Human Embryonic Kidney 293 cells.
Synonyms
Appearance
Sterile Filtered solution.
Purity
Zika NS1 protein is >90% pure as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The HEK derived recombinant Zika NS1 protein strain MR-766 (Prototype, Uganda, 1947, GenBank accession number AY632535).
The Zika NS1 protein is fused to a 6xHis tag at C-terminus and purified by proprietary chromatographic technique.

Product Specs

Introduction
Zika virus (ZIKV) is a member of the Flaviviridae family and the Flavivirus genus. It is primarily spread through the bites of infected Aedes mosquitoes, particularly Aedes aegypti and Aedes albopictus, which are active during daylight hours. Zika virus shares similarities with other flaviviruses such as dengue, yellow fever, Japanese encephalitis, and West Nile viruses. Like these viruses, Zika virus possesses an envelope, an icosahedral shape, and a non-segmented, single-stranded RNA genome of positive polarity. Zika fever, the infection caused by the virus, often presents with no or mild symptoms, resembling a mild form of dengue fever, and typically resolves with rest. However, since February 2016, there has been growing concern regarding the potential link between Zika fever during pregnancy and congenital disabilities in newborns. Evidence suggests that the virus can be transmitted from mother to fetus, potentially leading to adverse outcomes such as miscarriage or microcephaly, a condition characterized by underdeveloped head size. While the exact causal relationship between Zika virus and microcephaly is still under investigation, the association has raised significant public health concerns. Moreover, there is an established connection between Zika virus infection in adults and neurological complications, including Guillain-Barré syndrome.
Description
This product features recombinant Zika NS1 protein derived from HEK cells. It originates from the MR-766 strain (Prototype, Uganda, 1947), with the GenBank accession number AY632535. The Zika NS1 protein has been engineered with a 6xHis tag fused to its C-terminus and has undergone purification using a proprietary chromatographic method.
Physical Appearance
Clear, sterile solution.
Formulation
The Zika NS1 protein is supplied in a solution of Dulbecco's Phosphate Buffered Saline (DPBS) with a pH of 7.4.
Stability
For optimal storage, the Zika NS1 protein should be kept at -18°C or below. While it can remain stable at 4°C for up to one week, it is crucial to minimize freeze-thaw cycles to maintain protein integrity.
Purity
Analysis by SDS-PAGE has determined that the purity of the Zika NS1 protein is greater than 90%.
Applications
The optimal working titer of this product may vary depending on the specific application. It is recommended that each laboratory conduct appropriate optimization experiments to determine the most effective working concentration for their intended use.
Source
Human Embryonic Kidney 293 cells.

Product Science Overview

Introduction

The Zika virus (ZIKV) is a mosquito-borne flavivirus that has gained global attention due to its potential to cause severe congenital abnormalities and neurological complications. Among the various proteins encoded by the Zika virus, the non-structural protein 1 (NS1) plays a crucial role in viral replication and immune evasion. The recombinant NS1 protein, particularly when expressed in Human Embryonic Kidney (HEK) cells, has become a focal point of research due to its implications in understanding the virus’s pathogenicity and developing diagnostic tools and vaccines.

Structure and Function of NS1 Protein

The NS1 protein is a glycoprotein that exists in multiple forms, including a membrane-associated form and a secreted hexameric form. It is involved in various stages of the viral life cycle, including replication, immune modulation, and pathogenesis. The NS1 protein interacts with host cell components to facilitate viral replication and evade the host immune response. It also plays a role in the formation of replication complexes and the modulation of the host’s innate immune response.

Expression in HEK Cells

Human Embryonic Kidney (HEK) cells are commonly used for the expression of recombinant proteins due to their high transfection efficiency and ability to perform post-translational modifications similar to those in human cells. The expression of Zika NS1 protein in HEK cells allows for the production of a biologically active and properly folded protein, which is essential for studying its function and interactions.

Research and Applications
  1. Pathogenicity Studies: Research has shown that the NS1 protein from contemporary West African Zika virus strains enhances viral replication and reduces the activation of innate immune responses . This suggests that NS1 plays a significant role in the pathogenicity of the virus and its ability to cause severe disease.
  2. Vaccine Development: The NS1 protein is a target for vaccine development due to its immunogenic properties. Studies have explored the use of DNA vectors encoding NS1 for inducing protective immunity against Zika virus .
  3. Diagnostic Tools: The recombinant NS1 protein is used in the development of diagnostic assays for Zika virus infection. The presence of NS1 in the blood of infected individuals can be detected using immunoassays, providing a valuable tool for early diagnosis.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

Zika NS1 Paired Antibody
Mouse Anti Zika NS1 Paired
Cat. No.
BT10626
Source
Zika Ectodomain
Zika Ectodomain Recombinant
Cat. No.
BT10790
Source
Baculovirus Insect Cells.
Zika Envelope, sf9
Zika Envelope Recombinant, sf9
Cat. No.
BT11221
Source
Sf9, Baculovirus cells.
Zika NS1
Zika NS1 Protein Recombinant
Cat. No.
BT11286
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.