Visfatin Human

Visfatin is a dimeric type II phosphoribosyltransferase, which means it functions as an enzyme that catalyzes the transfer of phosphoribosyl groups. The recombinant human visfatin protein is typically expressed in Escherichia coli and purified to a high degree of purity (≥90%) for research purposes .

Visfatin has been implicated in several physiological and pathological processes:

1. Metabolic Regulation: Visfatin is upregulated in visceral fat and has been associated with insulin resistance. It was hypothesized to bind directly to the insulin receptor and exert insulin-like effects .
2. Immunoregulation: Visfatin plays a role in immune responses, including the regulation of neutrophil apoptosis and leukocyte cytokine production .
3. Inflammation: It has been shown to induce inflammatory genes in various tissues, including fetal membranes .

The role of visfatin in metabolic diseases such as obesity and diabetes has been a subject of extensive research. However, there are conflicting results regarding its association with these conditions. Some studies suggest that visfatin levels correlate with obesity and insulin resistance, while others do not find such associations .

Recombinant human visfatin is produced for research purposes to study its biological functions and potential therapeutic applications. The recombinant protein is often used in cell culture experiments to investigate its effects on various cell types. For example, it has been shown to induce VEGF secretion by PC-3 human prostate cancer cells .

Recombinant human visfatin is typically lyophilized and stored at -20 to -70°C to maintain its stability. It is reconstituted in sterile PBS before use in experiments .