Visfatin Human, His

Excess body fat is a major risk factor for developing insulin resistance and type 2 diabetes. Fat tissue releases proteins called adipocytokines, including leptin, adiponectin, resistin, tumor necrosis factor-α, and IL-6, which influence insulin sensitivity. These adipocytokines are believed to play a significant role in the development of insulin resistance, diabetes, unhealthy cholesterol levels, inflammation, and hardening of the arteries. However, the exact ways in which fat tissue leads to insulin resistance and the specific roles of adipocytokines in type 2 diabetes are not fully understood. Visfatin, also called pre-B cell colony-enhancing factor (PBEF), is primarily found in visceral fat. It was initially identified as a factor that works with IL-7 and stem cell factors to help B cell precursors grow. Visfatin-like proteins exist in various organisms, including fish, mollusks, bacteria, and vertebrates like humans and mice. It's suggested that visfatin plays a part in the body's natural defense system. Visfatin acts like insulin in a dose-dependent manner, meaning its effects increase with higher doses. It stimulates glucose uptake in muscles and fat cells and reduces glucose production in the liver, similar to insulin. Injecting mice with visfatin lowered their blood sugar levels, and it effectively reduced high blood sugar in diabetic mice lacking insulin. Visfatin binds to and activates the insulin receptor, leading to its phosphorylation and the activation of downstream signaling pathways. Interestingly, visfatin and insulin don't compete for the same binding site on the insulin receptor, implying they interact with distinct receptor regions. Therefore, visfatin may be involved in regulating blood sugar balance and could contribute to diabetes if its production or signaling is disrupted.

Recombinant Human Visfatin, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 511 amino acids. It has a molecular weight of 57 kDa. This recombinant human Visfatin is fused to a His tag at its N-terminus.

Sterile filtered solution with a concentration of 1 mg/ml.

Visfatin His tag protein is supplied in a solution containing 20mM Tris pH 8, 0.1mM DTT, and 10% glycerol.

For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Avoid repeated freeze-thaw cycles.

Purity exceeds 95.0% as determined by (a) RP-HPLC analysis and (b) SDS-PAGE analysis.

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Escherichia Coli.

MGSSHHHHHH SSGLVPRGSH MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG WNYILEKYDG HLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H.