Visfatin Mouse

Visfatin Mouse Recombinant
Cat. No.
BT30172
Source
Escherichia Coli.
Synonyms
PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.
Appearance
Sterile Filtered colorless 1mg/ml solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Visfatin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 1-491) containing a 20 aa His tag and having 511 amino acids. The total molecular mass is 57kDa. The Visfatin is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Visfatin, an adipocytokine primarily found in visceral fat, plays a crucial role in insulin sensitivity and glucose homeostasis. It exhibits insulin-mimetic effects, stimulating glucose uptake in muscles and adipocytes while suppressing glucose production in the liver. Visfatin achieves this by binding to and activating the insulin receptor, independent of insulin's binding site. This activation triggers downstream signaling pathways, mimicking insulin's actions. However, the complete understanding of how fat tissue contributes to insulin resistance and the precise role of adipocytokines, including visfatin, in the development of type 2 diabetes mellitus (T2DM) requires further investigation.
Description
Recombinant Mouse Visfatin, expressed in E. coli, is a purified protein with a molecular weight of 57 kDa. This non-glycosylated polypeptide chain encompasses amino acids 1-491 of the visfatin sequence, including a 20 amino acid His tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques, resulting in a highly pure (>90%) protein solution.
Physical Appearance
Clear, colorless solution, sterile filtered, at a concentration of 1 mg/ml.
Formulation
The Visfatin protein is supplied in a solution containing phosphate-buffered saline (PBS) at a pH of 7.4.
Stability
For short-term storage (2-4 weeks), store the Visfatin solution at 4°C. For extended storage, freeze the solution at -20°C. To ensure stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is recommended. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of the Visfatin protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECREKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERGWNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPITVATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHTVFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H.

Product Science Overview

Expression and Production

Recombinant mouse Visfatin is typically expressed in Escherichia coli and purified using conventional chromatography techniques . The recombinant protein is often produced with a high degree of purity, exceeding 95%, and is validated for use in various applications such as SDS-PAGE and functional studies .

Biological Properties

Visfatin exhibits several biological activities:

  • Insulin-Mimetic Effects: It mimics insulin effects in differentiating 3T3-L1 cells .
  • Cytokine Activity: As PBEF, it promotes B cell maturation and inhibits neutrophil apoptosis .
  • Hormonal Activity: It acts as a visceral-fat derived hormone that binds and activates the insulin receptor .
Tissue Distribution

Visfatin is predominantly found in visceral fat but is also present in other tissues. Its expression is regulated by various factors, including nutritional status and inflammatory signals.

Biological Functions

Visfatin plays a crucial role in several physiological processes:

  • Energy Metabolism: It is involved in the regulation of glucose metabolism and insulin sensitivity.
  • Immune Response: It modulates immune functions by promoting B cell maturation and inhibiting neutrophil apoptosis .
  • Cell Survival: It has been shown to enhance cell survival under stress conditions.
Modes of Action

Visfatin exerts its effects through multiple mechanisms:

  • Enzymatic Activity: As NAMPT, it catalyzes the conversion of nicotinamide to nicotinamide mononucleotide (NMN), a key step in NAD+ biosynthesis.
  • Receptor Binding: It binds to and activates the insulin receptor, mimicking the effects of insulin .
Regulatory Mechanisms

The expression and activity of Visfatin are regulated by various factors:

  • Nutritional Status: Its levels are influenced by the body’s nutritional state.
  • Inflammatory Signals: Inflammatory cytokines can modulate its expression.

In summary, recombinant mouse Visfatin is a versatile protein with significant roles in metabolism, immune response, and cell survival. Its recombinant form, produced in E. coli, is widely used in research to study its diverse biological functions and mechanisms of action.

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