Visfatin Antibody
Description
Product Specs
Product Science Overview
Visfatin, also known as pre-B cell colony-enhancing factor (PBEF) or nicotinamide phosphoribosyltransferase (NAMPT), is a multifunctional adipocytokine. It was initially identified as a protein secreted by visceral fat tissue and has since been found to play significant roles in various physiological and pathological processes, including inflammation, metabolism, and immune response .
Visfatin is involved in several critical biological functions:
- Insulin Mimetic Effects: Visfatin can bind to the insulin receptor and mimic insulin’s effects, thereby influencing glucose metabolism and insulin sensitivity .
- Inflammation and Immune Response: Visfatin is known to regulate inflammation and immune responses. It can activate human leukocytes and induce cytokine production, contributing to its proinflammatory and immunomodulating properties .
- Apoptosis Regulation: Visfatin plays a role in regulating apoptosis (programmed cell death) by modulating the expression of various genes and participating in signaling pathways such as the PI3K/AKT and Hippo pathways .
Mouse anti-human visfatin antibodies are monoclonal antibodies specifically designed to detect human visfatin in various experimental applications. These antibodies are produced by immunizing mice with human visfatin protein and then isolating the specific antibodies from the mouse’s immune system .
Applications
Mouse anti-human visfatin antibodies are widely used in research for:
- Western Blotting: To detect visfatin protein levels in different cell and tissue samples.
- ELISA (Enzyme-Linked Immunosorbent Assay): To quantify visfatin concentrations in biological samples.
- Immunohistochemistry: To visualize visfatin expression in tissue sections.
- Flow Cytometry: To analyze visfatin expression on the surface of cells .
Specificity and Reactivity
These antibodies are highly specific to human visfatin and do not cross-react with other proteins. They are also reactive with mouse and rat visfatin, making them versatile tools for studying visfatin in different species .
