TSHR Human
Escherichia Coli.
Sterile Filtered clear solution.
Protein is >85%.
Description
Recombinant Human Thyroid Stimulating Hormone Receptor produced in E. coli forms a dimer migrating at 46kDa on SDS-PAGE. TSHR Human contains multiple Leucine rich repeats with a histidine tag at N-terminus and purified by proprietary chromatographic technique.
Product Specs
Escherichia Coli.
Product Science Overview
The Thyroid Stimulating Hormone Receptor (TSHR) is a critical component of the hypothalamic-pituitary-thyroid axis, playing an essential role in regulating thyroid function. It is a Class A G protein-coupled receptor (GPCR) that mediates signaling through this axis. The receptor is activated by the thyroid-stimulating hormone (TSH), which is produced in the anterior pituitary gland. TSH controls both the morphological differentiation and the metabolic activity of thyroid cells .
Recombinant human TSHR (rhTSHR) is produced using genetic engineering techniques. The receptor is expressed in mammalian cell lines, such as Chinese Hamster Ovary (CHO) cells, through a lentiviral expression system. This method allows for the production of biologically active TSHR that can be used for various research and clinical applications .
The preparation of rhTSHR involves several steps:
- Expression: The human TSHR gene is introduced into mammalian cells, which then produce the receptor protein.
- Purification: The receptor is purified using techniques such as ion exchange chromatography, dye chromatography, and gel filtration chromatography. These methods ensure that the final product is highly pure and biologically active .
- Characterization: The purified receptor is characterized using various biochemical and biophysical techniques to confirm its structure and function. This includes methods like SDS polyacrylamide gel electrophoresis (SDS-PAGE), reverse phase high-performance liquid chromatography (HPLC), and size exclusion chromatography .
The TSHR is involved in several critical chemical reactions within the body. Upon binding to TSH, the receptor undergoes a conformational change that activates intracellular signaling pathways. This activation leads to the production of thyroid hormones, such as L-thyroxine (T4) and L-3,5,3′-triiodothyronine (T3). These hormones are essential for regulating metabolism, growth, and development .
Recombinant human TSHR has several clinical applications, particularly in the diagnosis and treatment of thyroid disorders. It is used to detect thyroglobulin release from metastatic thyroid cancer and to enhance radioiodine uptake into thyroid glands. Additionally, rhTSHR is used in the management of patients with thyroid cancer, helping to prepare them for whole-body iodide scans and serum thyroglobulin measurements .
