TSH Protein
HEK 293 cells.
Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Follitropin alpha chain, Follicle-stimulating hormone alpha chain, FSH-alpha, Lutropin alpha chain, Luteinizing hormone alpha chain, LSH-alpha, Thyrotropin alpha chain, Thyroid-stimulating hormone alpha chain, TSH-alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin alpha subunit, CG-alpha, Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, TSH-beta, TSH-B, Thyrotropin beta chain, Thyrotropin alfa.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
Description
Thyroid Stimulating Hormone Human Recombinant produced in HEK 293 cells.
Product Specs
Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Follitropin alpha chain, Follicle-stimulating hormone alpha chain, FSH-alpha, Lutropin alpha chain, Luteinizing hormone alpha chain, LSH-alpha, Thyrotropin alpha chain, Thyroid-stimulating hormone alpha chain, TSH-alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin alpha subunit, CG-alpha, Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, TSH-beta, TSH-B, Thyrotropin beta chain, Thyrotropin alfa.
HEK 293 cells.
Product Science Overview
TSH is a dimeric glycoprotein composed of two subunits: alpha (α) and beta (β). The α-subunit is common to other glycoprotein hormones like luteinizing hormone (LH) and follicle-stimulating hormone (FSH), while the β-subunit is unique to TSH and confers its biological specificity . The hormone binds to the thyroid-stimulating hormone receptor (TSHR) on the surface of thyroid cells, initiating a cascade of intracellular events that lead to the synthesis and secretion of thyroid hormones .
Recombinant human TSH (rhTSH), commercially known as Thyrogen™, is produced using recombinant DNA technology. This involves the insertion of human TSH genes into Chinese hamster ovary (CHO) cells, which are then cultured to produce the hormone . The recombinant form of TSH is used primarily for diagnostic purposes and as an adjunctive treatment in patients with thyroid cancer.
The production of rhTSH involves co-transfecting CHO cells with human α-subunit cDNA and a human β-subunit partial genomic clone. These cells are then cultured on microcarrier beads in serum-free conditions. The rhTSH-containing media is clarified and purified through a series of chromatographic techniques, including ion exchange, dye, and gel filtration chromatography . The final product is highly purified, with a purity level greater than 99%, and is biologically active .
rhTSH is primarily used in the management of thyroid cancer. It is administered to patients to stimulate the thyroid gland, allowing for the detection of metastatic lesions through radioactive iodine scanning or serum thyroglobulin testing . This approach is particularly beneficial for patients who cannot tolerate thyroid hormone withdrawal, which is traditionally required for these diagnostic procedures.
One of the main advantages of rhTSH is its ability to stimulate the thyroid gland without causing the symptoms of hypothyroidism that result from thyroid hormone withdrawal. However, rhTSH has relatively low affinity to the human TSH receptor compared to bovine or rodent TSH, which may limit its clinical efficacy in some cases . Future developments in TSH analogues with increased receptor affinity and potency are expected to enhance the effectiveness of this therapeutic approach .
