Toxoplasma P32
Description
Product Specs
For optimal stability, Toxoplasma P32 should be stored at -18°C. While the protein remains stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Product Science Overview
Toxoplasma gondii is an obligate intracellular protozoan parasite that causes toxoplasmosis, a disease with significant clinical implications, particularly in immunocompromised individuals and pregnant women. The parasite has a complex life cycle involving both definitive and intermediate hosts, and it can infect a wide range of warm-blooded animals, including humans .
The P32 antigen, also known as Dense Granule Antigen 6 (GRA6), is one of the many proteins secreted by Toxoplasma gondii. These dense granule proteins are released into the parasitophorous vacuole, a specialized compartment formed by the parasite within the host cell, and play crucial roles in modulating the host’s immune response and facilitating the parasite’s survival and replication .
Recombinant Toxoplasma gondii P32 (GRA6) is produced in Escherichia coli and contains 180 amino acids of the GRA6 protein. It is fused with a 6xHis tag at the C-terminus, which aids in its purification and detection. This recombinant protein forms dimers on SDS-PAGE under denatured conditions, and compared to other Toxoplasma monomer antigens, it exhibits better immunoreactivity .
GRA6 has shown strong immunogenicity in both mouse and human antibody profiles. It is recognized by the immune system of individuals infected with Toxoplasma gondii, making it a valuable antigen for the development of diagnostic assays and potential vaccines . The immunogenic properties of GRA6 have been leveraged in various studies to improve the sensitivity and specificity of serological tests for toxoplasmosis .
