T.gondii ROP4

Rhoptry proteins (ROPs) are a group of proteins secreted by the rhoptries, specialized organelles found in T. gondii. These proteins play crucial roles in the invasion and survival of the parasite within host cells. Among the identified rhoptry proteins, ROP1 and ROP2 have been studied extensively. ROP1 was initially thought to be essential for host cell invasion, but gene knockout studies revealed that it is not critical for invasion or intracellular survival .

The ROP2 family, which includes ROP2, ROP3/ROP8, and ROP4, was identified through cross-reacting monoclonal antibodies. ROP2, the founding member of this family, inserts into the parasitophorous vacuole membrane (PVM) and mediates the association between the PVM and host cell mitochondria. This interaction is crucial for the parasite’s invasion, intracellular replication, and rhoptry biogenesis .

The Toxoplasma Gondii ROP4 (RH2) Mosaic Recombinant is a recombinant protein derived from E. coli. This artificial mosaic protein contains the immunodominant regions of ROP4 (RH2) and is fused to Glutathione S-transferase (GST). The protein is highly purified, with a purity greater than 95% as determined by 10% PAGE (Coomassie staining) .

This recombinant protein is suitable for various applications, including ELISA and Western blots. It serves as an excellent antigen for detecting T. gondii with minimal specificity problems. The protein is immunoreactive with sera from T. gondii-infected individuals, making it a valuable tool for immunization protocols and generating antibodies that specifically target the protein of interest .

The T. gondii ROP4 (RH2) Mosaic Recombinant protein is used primarily in laboratory research. It is suitable for use in immunization protocols, generating specific antibodies, and detecting T. gondii in various assays. The protein is stable at 4°C for one week but should be stored below -18°C for long-term use .