T.gondii p40
Description
Product Specs
For optimal stability, T.gondii p40 should be stored at -18°C. While it can remain stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Product Science Overview
Toxoplasma gondii is an intracellular protozoan parasite responsible for toxoplasmosis, a widespread zoonotic infection. This parasite can infect nearly all warm-blooded animals, including humans. The infection is particularly concerning for immunocompromised individuals, pregnant women, and congenitally infected newborns .
Recombinant proteins are produced through genetic engineering techniques, where the gene encoding the protein of interest is inserted into an expression system, such as bacteria, yeast, or mammalian cells. For Toxoplasma gondii p40, the recombinant protein is typically produced in prokaryotic systems like Escherichia coli due to their efficiency and cost-effectiveness .
The production of recombinant Toxoplasma gondii p40 involves several steps:
- Gene Cloning: The gene encoding the p40 protein is cloned into an expression vector.
- Transformation: The vector is introduced into a host cell, commonly E. coli.
- Expression: The host cells are cultured under conditions that induce the expression of the p40 protein.
- Purification: The recombinant p40 protein is purified using techniques such as affinity chromatography to ensure high purity and yield .
The recombinant p40 protein has several applications:
- Serological Detection: It is used in diagnostic assays to detect antibodies against T. gondii in human and animal sera. Techniques like enzyme-linked immunosorbent assay (ELISA) utilize recombinant p40 to improve the sensitivity and specificity of toxoplasmosis diagnosis .
- Vaccine Development: The immunogenic properties of p40 make it a potential candidate for vaccine development. Research is ongoing to evaluate its efficacy in inducing protective immunity against T. gondii .
