T.gondii p29
Toxoplasma Gondii p29 (GRA7) Recombinant
Cat. No.
BT7384
Source
Escherichia Coli.
Synonyms
Appearance
Purity
Toxoplasma protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
The E.coli derived recombinant protein contains the p29 (GRA7) immunodominant regions, amino acids 24-100 and fused to a 26kDa GST tag.
Product Specs
Introduction
Toxoplasma gondii, a protozoan parasite, exhibits a complex life cycle with two distinct phases. The sexual phase exclusively occurs within members of the Felidae family (cats), the definitive hosts. Conversely, the asexual phase can transpire within a wide range of warm-blooded animals, including mammals and birds, serving as intermediate hosts. In these hosts, T. gondii invades cells, establishing intracellular vacuoles known as parasitophorous vacuoles. These vacuoles house bradyzoites, the slowly replicating form of the parasite. Primarily located in muscles and the brain, tissue cysts form within these vacuoles, effectively evading the host's immune system due to their intracellular location. While drug resistance can vary, complete eradication of these cysts remains challenging. Inside the vacuoles, T. gondii multiplies through binary fission until the host cell ruptures, releasing tachyzoites. These motile, asexually reproducing forms are generally cleared by the host's immune response. However, some tachyzoites may successfully infect new cells, differentiating into bradyzoites and perpetuating the infection.
Description
This recombinant protein, derived from E. coli, encompasses the immunodominant regions of the p29 protein (also known as GRA7) from Toxoplasma gondii. It spans amino acids 24 to 100 and is fused to a 26kDa GST tag.
Purity
The purity of the Toxoplasma protein exceeds 95%, as assessed by 10% SDS-PAGE followed by Coomassie blue staining.
Formulation
The protein is supplied in a buffer consisting of 50mM Tris-HCl, 1.5M urea, and 50% glycerol.
Stability
While the Toxoplasma protein remains stable at 4°C for up to one week, it is recommended to store it at temperatures below -18°C. Repeated freeze-thaw cycles should be avoided to maintain protein integrity.
Applications
This Toxoplasma antigen serves as a valuable tool for various immunological assays, including ELISA and Western blots. Its high specificity for Toxoplasma gondii makes it an excellent choice for the detection of this parasite.
Source
Escherichia Coli.
Purification Method
Toxoplasma protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of Toxoplasma gondii-infected individuals.
Product Science Overview
Characteristics of GRA7
- Molecular Weight: GRA7 has a molecular weight of 29 kDa .
- Expression: It is expressed in all three intraspecies subgroups of T. gondii and is associated with the parasite’s dense granules .
- Localization: In tachyzoite-infected cells, GRA7 accumulates within the PV and co-localizes with its delimiting membrane .
- Immunogenicity: GRA7 is immunoreactive with sera from humans with both acute and chronic T. gondii infections . It elicits a strong immune response, making it a valuable antigen for diagnostic purposes .
Recombinant GRA7
Recombinant GRA7 is produced using Escherichia coli as the expression system . The recombinant protein typically corresponds to amino acids 24-100 of the native GRA7 protein . It is purified using proprietary chromatographic techniques to achieve high purity levels (>95% as determined by SDS-PAGE) .
Applications:
- Diagnostic Use: Recombinant GRA7 is used as an antigen in various immunoassays, including ELISA and Western blots, for the detection of T. gondii infections . It has high sensitivity and specificity for IgG and IgM antibodies in human sera .
- Research: It is also used in research to study the immune response to T. gondii and to develop vaccines .
Storage and Handling:
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